Mouse Bmi1 ELISA Kit (V2LY-0626-LY2307)

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Datasheet
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Basic Information

Sensitivity
0.022 ng/mL
Detection Range
0.05-30 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
36.7 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
BMI1 Proto-Oncogene, Polycomb Ring Finger
Function
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373).
The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358).
In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).
Biological Process
Chromatin silencing Source: GO_Central
Hemopoiesis Source: UniProtKB
Histone H2A-K119 monoubiquitination Source: UniProtKB
Negative regulation of G0 to G1 transition Source: Reactome
Negative regulation of gene expression, epigenetic Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of fibroblast proliferation Source: BHF-UCL
Positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
Regulation of gene expression Source: BHF-UCL
Segment specification Source: ProtInc
Cellular Location
Nucleus; Cytoplasm
PTM
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

Fitieh, A., Locke, A. J., Motamedi, M., & Ismail, I. H. (2021). The Role of Polycomb Group Protein BMI1 in DNA Repair and Genomic Stability. International Journal of Molecular Sciences, 22(6), 2976.

Wu, D., He, X., Wang, W., Hu, X., Wang, K., & Wang, M. (2020). Long noncoding RNA SNHG12 induces proliferation, migration, epithelial–mesenchymal transition, and stemness of esophageal squamous cell carcinoma cells via post‐transcriptional regulation of BMI1 and CTNNB1. Molecular oncology, 14(9), 2332-2351.

Solorzano-Vargas, R. S., Bjerknes, M., Wu, S. V., Wang, J., Stelzner, M., Dunn, J. C., ... & Martín, M. G. (2019). The cellular regulators PTEN and BMI1 help mediate NEUROGENIN-3–induced cell cycle arrest. Journal of Biological Chemistry, 294(41), 15182-15192.

Wu, L., Zhang, D., Zhou, L., Pei, Y., Zhuang, Y., Cui, W., & Chen, J. (2019). FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis. EBioMedicine, 41, 384-394.

Li, P., Liu, D., Mei, Q., Wang, X., Zheng, Y., Pan, H., & Zheng, L. (2018, June). Research on the Effect of BMI1 Gene Silencing on the Proliferation Activity of Human Breast Cancer Cells. In 2018 8th International Conference on Mechatronics, Computer and Education Informationization (MCEI 2018) (pp. 88-93). Atlantis Press.

Vaishnave, S. (2018). BMI1 and PTEN are key determinants of breast cancer therapy: A plausible therapeutic target in breast cancer. Gene, 678, 302-311.

Koh, H., Park, H., Chandimali, N., Huynh, D. L., Zhang, J. J., Ghosh, M., ... & Jeong, D. K. (2017). MicroRNA-128 suppresses paclitaxel-resistant lung cancer by inhibiting MUC1-C and BMI-1 in cancer stem cells. Oncotarget, 8(66), 110540.

Liu, X., Wei, W., Li, X., Shen, P., Ju, D., Wang, Z., ... & Xi, R. (2017). BMI1 and MEL18 promote colitis-associated cancer in mice via REG3B and STAT3. Gastroenterology, 153(6), 1607-1620.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human BMI1 ELISA Kit (CAT#: V2LY-0626-LY3881)

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Isotype control

For research use only. Not intended for any clinical use.

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