What applications has the Rat Anti-AChR Recombinant Antibody (V2-12500) been tested in?

This antibody is validated for use in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, and Flow Cytometry.

What is the immunogen sequence for this antibody?

For proprietary reasons, we do not disclose the specific immunogen sequence for our antibodies. However, this recombinant antibody is designed to recognize the alpha subunit of the acetylcholine receptor. For more detailed information, please contact our technical support team.

Rat Anti-AChR Recombinant Antibody (V2-12500) (CBMAB-0990-CN)

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Published Data

Rat Anti-AChR Recombinant Antibody (Mab35) (CBMAB-0990-CN) in IF

Nonfunctional AChRs in fast muscles of the love sofa mutant. A, Love sofa larvae were doubly labeled with mAb35 antibody (green). ...View More

Park, J. Y., Mott, M., Williams, T., Ikeda, H., Wen, H., Linhoff, M., & Ono, F. (2014). A single mutation in the acetylcholine receptor δ-subunit causes distinct effects in two types of neuromuscular synapses. Journal of Neuroscience, 34(31), 10211-10218.

Rat Anti-AChR Recombinant Antibody (Mab35) (CBMAB-0990-CN) in FC

MITC cultures were untreated or incubated with IFN- for 3 days. AChR surface expression was analyzed by flow cytometry using mAb directed against -AChR subunit. ...View More

Poëa-Guyon, S., Christadoss, P., Le Panse, R., Guyon, T., De Baets, M., Wakkach, A., ... & Berrih-Aknin, S. (2005). Effects of cytokines on acetylcholine receptor expression: implications for myasthenia gravis. The Journal of Immunology, 174(10), 5941-5949.

Datasheet

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Basic Information

Host Animal

Rat

Clone

V2-12500

Application

ICC, IF

Immunogen

Electrophorus electricus acetylcholine receptor

Specificity

Human, Rat, Fish

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Endotoxin

<2 EU/mg

Format

Liquid

Buffer

PBS, pH 7.0

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C. Do not freeze. Store In the Dark.

More Infomation

Target

Full Name

acetylcholine receptor

Introduction

AChR (Acetylcholine receptor) is an integral membrane protein that responds to the binding of acetylcholine, a neurotransmitter.

Alternative Names

Acetylcholine Receptor

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Rat Anti-AChR Recombinant Antibody (V2-1553) (CAT#: CBMAB-0948-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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