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USP4

The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene.
Full Name
ubiquitin specific peptidase 4 (proto-oncogene)
Function
Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins (PubMed:16316627, PubMed:16472766, PubMed:16339847, PubMed:20595234, PubMed:22347420, PubMed:25404403, PubMed:28604766).
Deubiquitinates PDPK1 (PubMed:22347420).
Deubiquitinates TRIM21 (PubMed:16316627).
Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface (PubMed:16339847).
Deubiquitinates HAS2 (PubMed:28604766).
May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3 (PubMed:20595234).
This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP (PubMed:20595234).
May also play a role in the regulation of quality control in the ER (PubMed:16339847).
Biological Process
Biological Process negative regulation of protein ubiquitination Source:UniProtKB1 Publication
Biological Process protein deubiquitination Source:UniProtKB3 Publications
Biological Process protein localization to cell surface Source:UniProtKB1 Publication
Biological Process regulation of protein stability Source:UniProtKB1 Publication
Biological Process spliceosomal tri-snRNP complex assembly Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro
Cellular Location
Cytoplasm
Nucleus
Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type.
PTM
Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated.

Anti-USP4 antibodies

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Target: USP4
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 5E12
Application*: E, WB
Target: USP4
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBXU-021
Application*: WB, IC
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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