Rat Anti-CTSB Recombinant Antibody (CBYY-C2310) (V2LY-0125-LY400)
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Published Data
Rat Anti-CTSB Recombinant Antibody (CBYY-C2310) (V2LY-0125-LY400) in Immunoblotting
Immunoblot analysis of pancreas homogenates shows an increased expression of cathepsin B and L in the Rab7 knockout mice.
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Zierke, L., John, D., Gischke, M., Tran, Q. T., Sendler, M., Weiss, F. U., ... & Aghdassi, A. A. (2024). Initiation of acute pancreatitis in mice is independent of fusion between lysosomes and zymogen granules. Cellular and Molecular Life Sciences, 81(1), 207. Distributed under Open Access license CC BY 4.0, without modification.
Rat Anti-CTSB Recombinant Antibody (CBYY-C2310) (V2LY-0125-LY400) in Immunoblotting
Immunoblots of pancreatic subcellular fractions of WT mice (C57BL/6) show a redistribution of lysosomal proteins LAMP1, LAMP2, CTSB, CTSC from the lysosomal enriched fraction into the cytosolic fraction after GPN injection.
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Zierke, L., John, D., Gischke, M., Tran, Q. T., Sendler, M., Weiss, F. U., ... & Aghdassi, A. A. (2024). Initiation of acute pancreatitis in mice is independent of fusion between lysosomes and zymogen granules. Cellular and Molecular Life Sciences, 81(1), 207. Distributed under Open Access license CC BY 4.0, without modification.
Datasheet
Target
References
Q & As
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Protocols
Associated Products
Basic Information
Host Animal
Rat
Clone
CBYY-C2310
Application
WB, IF
Immunogen
Recombinant mouse Cathepsin B, His18-Phe339.
Host Species
Rat
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
| Application | Note |
| WB | 1 μg/mL |
Formulations & Storage [For reference only, actual COA shall prevail!]
Format
Lyophilized
Buffer
PBS & Trehalose
Preservative
None
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation
Target
Full Name
Cathepsin B
Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:12220505).
Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505).
Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity).
Has also been implicated in tumor invasion and metastasis (PubMed:3972105).
Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505).
Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity).
Has also been implicated in tumor invasion and metastasis (PubMed:3972105).
Biological Process
Cellular response to thyroid hormone stimulus Source: UniProtKB
Collagen catabolic process Source: BHF-UCL
Epithelial cell differentiation Source: UniProtKB
Neutrophil degranulation Source: Reactome
Proteolysis Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: BHF-UCL
Regulation of apoptotic process Source: UniProtKB
Regulation of catalytic activity Source: InterPro
Toll-like receptor signaling pathway Source: Reactome
Collagen catabolic process Source: BHF-UCL
Epithelial cell differentiation Source: UniProtKB
Neutrophil degranulation Source: Reactome
Proteolysis Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: BHF-UCL
Regulation of apoptotic process Source: UniProtKB
Regulation of catalytic activity Source: InterPro
Toll-like receptor signaling pathway Source: Reactome
Cellular Location
Lysosome; Apical cell membrane; Extracellular space; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells (By similarity).
Involvement in disease
Keratolytic winter erythema (KWE):
The gene represented in this entry is involved in disease pathogenesis. Tandem duplications in a non-coding genomic region containing an active enhancer element for CTSB result in CTSB abnormal expression with pathological consequences.
An autosomal dominant genodermatosis characterized by recurrent episodes of palmoplantar erythema and epidermal peeling presenting seasonal variation. KWE manifests during childhood. Skin lesions may spread to the dorsum of hands and feet and to the interdigital spaces. Lower legs, knees and thighs may also be involved. A less common finding is a slowly migratory, annular erythema that is seen mostly on the extremities. Between flares, the skin can appear unremarkable. Itching can occur, and hyperhidrosis, associated with a pungent odor, is invariably present. Formation of vesicles is rare, whereas keratolysis that causes the formation of dry blisters is regularly seen. Cold weather, moisture, febrile diseases, and physical and mental stress can trigger exacerbations. In severely affected individuals, skin manifestations persist unremittingly. Penetrance of the disease is high, but expressivity is variable, even within the same family.
The gene represented in this entry is involved in disease pathogenesis. Tandem duplications in a non-coding genomic region containing an active enhancer element for CTSB result in CTSB abnormal expression with pathological consequences.
An autosomal dominant genodermatosis characterized by recurrent episodes of palmoplantar erythema and epidermal peeling presenting seasonal variation. KWE manifests during childhood. Skin lesions may spread to the dorsum of hands and feet and to the interdigital spaces. Lower legs, knees and thighs may also be involved. A less common finding is a slowly migratory, annular erythema that is seen mostly on the extremities. Between flares, the skin can appear unremarkable. Itching can occur, and hyperhidrosis, associated with a pungent odor, is invariably present. Formation of vesicles is rare, whereas keratolysis that causes the formation of dry blisters is regularly seen. Cold weather, moisture, febrile diseases, and physical and mental stress can trigger exacerbations. In severely affected individuals, skin manifestations persist unremittingly. Penetrance of the disease is high, but expressivity is variable, even within the same family.
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For research use only. Not intended for any clinical use.
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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