Mouse Anti-CTSB Recombinant Antibody (CBYY-C2311) (V2LY-0125-LY401)

Name: Mouse Anti-CTSB Recombinant Antibody (CBYY-C2311)
SKU: V2LY-0125-LY401
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CTSB Recombinant Antibody (CBYY-C2311) (V2LY-0125-LY401) in WB

LPS-primed J774A.1 macrophages were incubated with CA for 0.5 h before S. sonnei infection for an additional 21 h. The detectable levels of pro-cathepsin B and mature cathepsin B in the cell lysates (A) and in the supernatants (B) were analysed by Western blotting. ...View More

Hua, K. F., Lin, Y. B., Chiu, H. W., Wong, W. T., Ka, S. M., Wu, C. H., ... & Li, L. H. (2024). Cinnamaldehyde inhibits the NLRP3 inflammasome by preserving mitochondrial integrity and augmenting autophagy in Shigella sonnei-infected macrophages. Journal of Inflammation, 21(1), 18. Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Mouse Anti-CTSB Recombinant Antibody (CBYY-C2311) (V2LY-0125-LY401) in WB

Effects of AME treatment on hepatokines and myokines associated with neuroprotection in the liver (A), skeletal muscle (B), plasma (C), and hippocampus (D) in T2DM mice. Values are means ± SEM. There is no significant difference between values with the same superscript letters within groups (n = 9 per group). ...View More

Lee, H., Kim, S. Y., & Lim, Y. (2023). Annona muricate Extract Supplementation Contributes to Improve Aberrant Multi-Organ Energy Metabolism via Muscle-Brain Connectivity in Diabetic Mice. Nutrients, 15(11), 2559. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTSB Recombinant Antibody (CBYY-C2311) (V2LY-0125-LY401) in Immunoblotting

Western Blot analysis of cathepsin B (CTSB), intersectin-1 (ITSN1), syntaxin 4 (STX4), HRas, PIK3C2A, and DIS3 in samples from the two groups under study (STEAP1 siRNA versus scramble siRNA). Normalization of the protein input was performed using the stain-free gel. Results are expressed as fold variation expression to the scramble siRNA group and representative immunoblots are shown in the right panel. Error bars indicate mean ± SEM (n = 3 in duplicate). ...View More

Rocha, S. M., Santos, F. M., Socorro, S., Passarinha, L. A., & Maia, C. J. (2023). Proteomic analysis of STEAP1 knockdown in human LNCaP prostate cancer cells. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1870(7), 119522. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTSB Recombinant Antibody (CBYY-C2311) (V2LY-0125-LY401) in WB

After Mn exposure (MnCl2, 30 mg/kg, intranasal instillation, daily for 3 weeks), striatum and midbrain tissues were analyzed for the protein levels of NLRP3, cleaved CASP1, active CTSB, and LAMP1 by western blotting. ...View More

Pajarillo, E., Kim, S., Digman, A., Dutton, M., Son, D. S., Aschner, M., & Lee, E. (2023). The role of microglial LRRK2 kinase in manganese-induced inflammatory neurotoxicity via NLRP3 inflammasome and RAB10-mediated autophagy dysfunction. Journal of Biological Chemistry, 299(7). Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTSB Recombinant Antibody (CBYY-C2311) (V2LY-0125-LY401) in IF

After transfection of LRRK2 WT and G2019S vectors following Mn exposure, BV2 cells were analyzed for the fluorescence intensity and colocalization of p-RAB10, NLRP3, and CTSB in microglia by immunofluorescence. ...View More

Mouse Anti-CTSB Recombinant Antibody (CBYY-C2311) (V2LY-0125-LY401) in IF

Representative images of cathepsin B (red) labeled with Alexa Fluor 594 in MRC-5 cells treated with P25 NPs and Fe(1%)-N doped P25 NPs (10 and 50 μg/mL) at 72 h of exposure. White arrows indicate the vesicular disposition of cathepsin B in control cells. Nuclei (blue) were stained with DAPI. Scale bar: 20 µm. ...View More

Miu, B. A., Voinea, I. C., Diamandescu, L., & Dinischiotu, A. (2023). MRC-5 human lung fibroblasts alleviate the genotoxic effect of Fe-N Co-doped titanium dioxide nanoparticles through an OGG1/2-dependent reparatory mechanism. International Journal of Molecular Sciences, 24(7), 6401. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTSB Recombinant Antibody (CBYY-C2311) (V2LY-0125-LY401) in Immunoblotting

Immunoblots for mitochondrial proteins in BMM WT, CtsB KO, CtsL KO and CtsS KO stimulated with 0.5 mM LLOMe for 1 h. (repeated three times with similar results). ...View More

Bussi, C., Heunis, T., Pellegrino, E., Bernard, E. M., Bah, N., Dos Santos, M. S., ... & Gutierrez, M. G. (2022). Lysosomal damage drives mitochondrial proteome remodelling and reprograms macrophage immunometabolism. Nature Communications, 13(1), 7338. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C2311

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-339 representing full length cathepsin B of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cathepsin B

Entrez Gene ID

Human	1508
Mouse	13030

UniProt ID

Human	P07858
Mouse	P10605

Function

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:12220505).

Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505).

Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity).

Has also been implicated in tumor invasion and metastasis (PubMed:3972105).

Biological Process

Cellular response to thyroid hormone stimulus Source: UniProtKB
Collagen catabolic process Source: BHF-UCL
Epithelial cell differentiation Source: UniProtKB
Neutrophil degranulation Source: Reactome
Proteolysis Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: BHF-UCL
Regulation of apoptotic process Source: UniProtKB
Regulation of catalytic activity Source: InterPro
Toll-like receptor signaling pathway Source: Reactome

Cellular Location

Lysosome; Apical cell membrane; Extracellular space; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells (By similarity).

Involvement in disease

Keratolytic winter erythema (KWE):
The gene represented in this entry is involved in disease pathogenesis. Tandem duplications in a non-coding genomic region containing an active enhancer element for CTSB result in CTSB abnormal expression with pathological consequences.
An autosomal dominant genodermatosis characterized by recurrent episodes of palmoplantar erythema and epidermal peeling presenting seasonal variation. KWE manifests during childhood. Skin lesions may spread to the dorsum of hands and feet and to the interdigital spaces. Lower legs, knees and thighs may also be involved. A less common finding is a slowly migratory, annular erythema that is seen mostly on the extremities. Between flares, the skin can appear unremarkable. Itching can occur, and hyperhidrosis, associated with a pungent odor, is invariably present. Formation of vesicles is rare, whereas keratolysis that causes the formation of dry blisters is regularly seen. Cold weather, moisture, febrile diseases, and physical and mental stress can trigger exacerbations. In severely affected individuals, skin manifestations persist unremittingly. Penetrance of the disease is high, but expressivity is variable, even within the same family.

Dai, J., Zhang, Q., Wan, C., Liu, J., Zhang, Q., Yu, Y., & Wang, J. (2021). Significances of viable synergistic autophagy-associated cathepsin B and cathepsin D (CTSB/CTSD) as potential biomarkers for sudden cardiac death. BMC Cardiovascular Disorders, 21(1), 1-15.

Iwama, H., Mehanna, S., Imasaka, M., Hashidume, S., Nishiura, H., Yamamura, K. I., ... & Ohmuraya, M. (2021). Cathepsin B and D deficiency in the mouse pancreas induces impaired autophagy and chronic pancreatitis. Scientific reports, 11(1), 1-10.

Mizunoe, Y., Kobayashi, M., Hoshino, S., Tagawa, R., Itagawa, R., Hoshino, A., ... & Higami, Y. (2020). Cathepsin B overexpression induces degradation of perilipin 1 to cause lipid metabolism dysfunction in adipocytes. Scientific reports, 10(1), 1-12.

Kuang, F., Liu, J., Li, C., Kang, R., & Tang, D. (2020). Cathepsin B is a mediator of organelle-specific initiation of ferroptosis. Biochemical and Biophysical Research Communications, 533(4), 1464-1469.

Ni, Y., Hai, Z., Zhang, T., Wang, Y., Yang, Y., Zhang, S., & Liang, G. (2019). Cathepsin B Turning Bioluminescence “On” for Tumor Imaging. Analytical Chemistry, 91(23), 14834-14837.

Aghdassi, A. A., John, D. S., Sendler, M., Weiss, F. U., Reinheckel, T., Mayerle, J., & Lerch, M. M. (2018). Cathepsin D regulates cathepsin B activation and disease severity predominantly in inflammatory cells during experimental pancreatitis. Journal of Biological Chemistry, 293(3), 1018-1029.

Sendler, M., Weiss, F. U., Golchert, J., Homuth, G., van den Brandt, C., Mahajan, U. M., ... & Mayerle, J. (2018). Cathepsin B-mediated activation of trypsinogen in endocytosing macrophages increases severity of pancreatitis in mice. Gastroenterology, 154(3), 704-718.

Araujo, T. F., Cordeiro, A. V., Vasconcelos, D. A., Vitzel, K. F., & Silva, V. R. (2018). The role of cathepsin B in autophagy during obesity: A systematic review. Life sciences, 209, 274-281.

Bai, H., Yang, B., Yu, W., Xiao, Y., Yu, D., & Zhang, Q. (2018). Cathepsin B links oxidative stress to the activation of NLRP3 inflammasome. Experimental cell research, 362(1), 180-187.

Yan, Y., Zhou, K., Wang, L., Wang, F., Chen, X., & Fan, Q. (2017). Clinical significance of serum cathepsin B and cystatin C levels and their ratio in the prognosis of patients with esophageal cancer. OncoTargets and therapy, 10, 1947.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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