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Mouse Angiogenesis Antibody Array (Membrane, 53 Targets) (Y0234) (AbAr-0234-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Mouse Angiogenesis Antibody Array detects 53 mouse angiogenesis-related proteins simultaneously.
Target Array
Angiogenesis Array
ADAMTS1, Endothelin-1, NOV/CCN3/IGFBP-9, Amphiregulin, FGF acidic, Osteopontin, Angiogenin, FGF basic, PD-ECGF, Angiopoietin-1, FGF-7/KGF, PDGF-AA, Angiopoietin-3, Fractalkine, PDGF-AB/BB, CCL2/JE/MCP-1, GM-CSF, Pentraxin-3, CCL3/MIP-1 alpha, HB-EGF, PlGF-2, CXCL1/KC, HGF, Prolactin, CXCL10/IP-10/CRG-2, IGFBP-1, Proliferin, CXCL12/SDF-1, IGFBP-2, Serpin E1/PAI-1, CXCL16, IGFBP-3, Serpin F1/PEDF, CXCL4/PF4, IL-1 alpha, Thrombospondin-2, Cyr61/CNN1/IGFBP-10, IL-1 beta, TIMP-1, DLL4, IL-10, TIMP-4, DPPIV/CD26, Leptin, Coagulation Factor III, EGF, MMP-3 (pro, mature), VEGF, Endoglin/CD105, MMP-8 (pro), VEGF-B, Endostatin/Collagen XVIII, MMP-9 (pro, active)
Species Reactivity
Sample Type
Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Serum
Multiplex Protein Detection
Solid Support
Detection Assay
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Angiogenesis is the formation of new blood vessels. This process involves the migration, growth, and differentiation of endothelial cells, which line the inside wall of blood vessels. The process of angiogenesis is controlled by chemical signals in the body.
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For Research Use Only. Not For Clinical Use.