What is the primary specificity of the Mouse Anti-A. flavus Aflatoxin AFB1 Recombinant Antibody (V2-503388)?

This recombinant antibody is highly specific for Aflatoxin B1 (AFB1), which is considered the most potent and prevalent naturally occurring mycotoxin. While it may show some minimal cross-reactivity with closely related aflatoxin analogs, its primary binding affinity is optimized for robust and accurate detection of AFB1.

What are the best storage conditions for this antibody to ensure long-term stability?

For optimal long-term preservation, we recommend storing the Mouse Anti-A. flavus Aflatoxin AFB1 Recombinant Antibody (V2-503388) in aliquots at -20°C or -80°C. Avoid repeated freeze-thaw cycles, as this can degrade antibody activity. For short-term storage (up to a few weeks), 2-8°C is generally acceptable. Always consult the product datasheet for the most precise storage and handling recommendations.

Mouse Anti-A. flavus Aflatoxin AFB1 Recombinant Antibody (V2-503388) (CBMAB-V208-1363-FY)

See all A. flavus Aflatoxin AFB1 antibodies

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Published Data

Mouse Anti-A. flavus Aflatoxin AFB1 Recombinant Antibody (CBMW-H1193) (CBMAB-V208-1363-FY) in IHC

AFB1 staining in human cirrhotic tissue. Representative cases of AFB1adducts immunostaining in human cirrhotic nodules with AFB1 positive hepatocytes were surrounded by septa with positive cells. ...View More

Gramantieri, L., Gnudi, F., Vasuri, F., Mandrioli, D., Fornari, F., Tovoli, F., ... & Giovannini, C. (2022). Aflatoxin B1 DNA-adducts in hepatocellular carcinoma from a low exposure area. Nutrients, 14(8), 1652.

Mouse Anti-A. flavus Aflatoxin AFB1 Recombinant Antibody (CBMW-H1193) (CBMAB-V208-1363-FY) in WB

Western blot analysis of co-IPs to assess the binding between AHR and AFB1. ...View More

Zhu, Q., Ma, Y., Liang, J., Wei, Z., Li, M., Zhang, Y., ... & Jiao, Y. (2021). AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma. Signal Transduction and Targeted Therapy, 6(1), 299.

Mouse Anti-A. flavus Aflatoxin AFB1 Recombinant Antibody (CBMW-H1193) (CBMAB-V208-1363-FY) in ELISA

AFB1-DNA adducts formation in AFB1-treated SMMC-7721 cells with overexpression of miR-429 (see Section 2). Levels of AFB1-DNA adducts were tested using comparative ELISA. Data were analyzed from three independent tests using one-way ANOVA with Bonferroni corrections. *P < 0.05. ...View More

Huang, X. Y., Yao, J. G., Huang, H. D., Wang, C., Ma, Y., Xia, Q., & Long, X. D. (2013). MicroRNA-429 modulates hepatocellular carcinoma prognosis and tumorigenesis. Gastroenterology research and practice, 2013.

Mouse Anti-A. flavus Aflatoxin AFB1 Recombinant Antibody (CBMW-H1193) (CBMAB-V208-1363-FY) in Dot Blot

HCT-8 cells stably-transfected withempty vector or shCYP3A5 were treated with DMSO or 10 mM AFB1 for 72 h. AFB1 DNA adduct were detected using immunodot-blot assay. DNA adduct measured by multi gauge software (bottom panel), respectively. ...View More

Kim, J., Park, S. H., Do, K. H., Kim, D., & Moon, Y. (2016). Interference with mutagenic aflatoxin B1-induced checkpoints through antagonistic action of ochratoxin A in intestinal cancer cells: A molecular explanation on potential risk of crosstalk between carcinogens. Oncotarget, 7(26), 39627.

Datasheet

Summary

Host Animal

Mouse

Specificity

A. flavus

Clone

V2-503388

Antibody Isotype

IgG1, κ

Application

ELISA, ICC, IF, IHC-Fr, IHC-P, WB

Basic Information

Immunogen

Aflatoxin B1 DNA (against the midazole ring-opened persistent form of the major N-7 guanine adduct of AFB1).

Specificity

A. flavus

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000
IF(ICC)	1:100
ELISA	5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.1% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Aspergillus Flavus Aflatoxin Afb1

Introduction

Aflatoxins are poisonous carcinogens that are produced by certain molds (Aspergillus flavus and Aspergillus parasiticus) which grow in soil, decaying vegetation, hay, and grains.

Alternative Names

Aflatoxin B1

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-A. flavus Aflatoxin AFB1 Recombinant Antibody (V2-503392)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote
Second antibody and isotype control

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