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Mouse Anti-AOX1 Recombinant Antibody (D-8) (CBMAB-1084-CN)

This product is a mouse antibody that recognizes AOX1 of human. The antibody D-8 can be used for immunoassay techniques such as: WB, ELISA, IF, IHC-P, IP.
See all AOX1 antibodies
Published Data
Mouse Anti-AOX1 Recombinant Antibody (D-8) in WB
Primary human skeletal muscle cells (5 × 105 cells) were plated in a 60-mm dish. Cell lysate was collected before (undifferentiated myoblasts or UD) and 1 (D1), 3 (D3) and 5 (D5) days after differentiation. The expressions of aldehyde oxidase was measured by western blots. Data are mean ± SEM (n = 4-6). *P < 0.05 compared with undifferentiated cells (UD) and tested by ANOVA with Tukey's multiple comparison. ...View More
Srihirun, S., Park, J. W., Teng, R., Sawaengdee, W., Piknova, B., & Schechter, A. N. (2020). Nitrate uptake and metabolism in human skeletal muscle cell cultures. Nitric Oxide, 94, 1-8.
Mouse Anti-AOX1 Recombinant Antibody (D-8) in WB
Western blots of the neuronal nitic oxide synthase (NOS-1), xanthine oxidoreductase (XOR), aldehyde oxidase (AO) and the nitrate transporter, sialin, in human skeletal muscle tissue of each of thirteen individual participants
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal standard. Note that all proteins were present in human skeletal muscle, and that there was large inter-individual variation in all four proteins, most notably in sialin and XOR. ...View More
Wylie, L. J., Park, J. W., Vanhatalo, A., Kadach, S., Black, M. I., Stoyanov, Z., ... & Piknova, B. (2019). Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise. The Journal of physiology, 597(23), 5565-5576.

Summary

Host Animal
Mouse
Specificity
Human
Clone
D-8
Antibody Isotype
IgG1, κ
Application
WB, ELISA, IF, IHC-P, IP

Basic Information

Immunogen
Amino acids 854-917 mapping within an internal region of AOX1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aldehyde Oxidase 1
Introduction
Aldehyde oxidase produces hydrogen peroxide and, under certain conditions, can catalyze the formation of superoxide. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. The protein plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents.
Entrez Gene ID
316
UniProt ID
Q06278
Alternative Names
AO; AOH1
Function
Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis.
Biological Process
Drug metabolic process Source: UniProtKB
Vitamin B6 metabolic process Source: Reactome
Xanthine catabolic process Source: GO_Central
Cellular Location
Cytoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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