Sign in or Register   Sign in or Register
  |  

Mouse Anti-ATP5F1E Recombinant Antibody (2F3) (CBMAB-A0627-LY)

The product is antibody recognizes ATP5E. The antibody 1A8 immunoassay techniques such as: WB, ELISA.
See all ATP5F1E antibodies
Published Data
Mouse Anti-ATP5F1E Recombinant Antibody (2F3) in IHC
Representative scores for ATP5E IHC staining in CRC patients. ...View More
Huang, Y. J., Jan, Y. H., Chang, Y. C., Tsai, H. F., Wu, A. T., Chen, C. L., & Hsiao, M. (2019). ATP synthase subunit epsilon overexpression promotes metastasis by modulating AMPK signaling to induce epithelial-to-mesenchymal transition and is a poor prognostic marker in colorectal cancer patients. Journal of Clinical Medicine, 8(7), 1070.
Mouse Anti-ATP5F1E Recombinant Antibody (2F3) in WB
Western blot analysis of E-cadherin and Snail expressions upon ATP5E knockdown and overexpression. ...View More
Huang, Y. J., Jan, Y. H., Chang, Y. C., Tsai, H. F., Wu, A. T., Chen, C. L., & Hsiao, M. (2019). ATP synthase subunit epsilon overexpression promotes metastasis by modulating AMPK signaling to induce epithelial-to-mesenchymal transition and is a poor prognostic marker in colorectal cancer patients. Journal of Clinical Medicine, 8(7), 1070.
Mouse Anti-ATP5F1E Recombinant Antibody (2F3) in WB
Patient mitochondria accumulate Fo subunit c of ATP synthase. SDS-PAGE and WB analysis of indicated ATP synthase subunits in the isolated fibroblast mitochondria (A) and fibroblasts (B) from control (C), patient with the ATP5E mutation (P) and two patients with a mutation in the TMEM70 gene (PTM70). (C) Accumulated Fo c subunits resist to DDM solubilization. Isolated mitochondria from patient and control fibroblasts were solubilized with DDM (4 g/g protein) and 29 000g supernatant (Sup) and pellet (Pel) were analyzed for the content of subunits F1 a and Fo c. (D) Pellet from the patient accumulates Fo c but not the subunits Fo a or Fo d. ...View More
Mayr, J. A., Havlíčková, V., Zimmermann, F., Magler, I., Kaplanova, V., Ješina, P., ... & Houštěk, J. (2010). Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 ε subunit. Human molecular genetics, 19(17), 3430-3439.

Summary

Host Animal
Mouse
Specificity
Human
Clone
2F3
Antibody Isotype
IgG1, κ
Application
WB, ELISA, IHC

Basic Information

Immunogen
ATP5E (AAH01690, 1 a.a. ~ 51 a.a) full-length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ATP Synthase F1 Subunit Epsilon
Introduction
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13. [provided by RefSeq]
Entrez Gene ID
514
UniProt ID
P56381
Alternative Names
ATPE; MGC104243
Function
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits (By similarity).
Biological Process
ATP biosynthetic process Source: Reactome
Cristae formation Source: Reactome
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Cellular Location
Mitochondrion; Mitochondrion inner membrane
Involvement in disease
Mitochondrial complex V deficiency, nuclear type 3 (MC5DN3): A mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ATP5F1E Recombinant Antibody (2F3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare