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Mouse Anti-Biotin Recombinant Antibody (BK-1/39) (CBMAB-XB0095-YC)

Provided herein is a Mouse Recombinant Antibody against Biotin. The antibody can be used for immunoassay techniques, such as IHC.
See all Biotin antibodies
Published Data
Fig.1 Quantification of sortase labeling of Kell-LPETGG RBCs with different biotin-containing peptides by immunoblotting (IB) using streptavidin-HRP.
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Fig.2 Flow cytometric analysis (forward scatter versus GFP) of B cell subsets in a cmb1 mouse at day 20 after tamoxifen treatment.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
BK-1/39
Antibody Isotype
IgG1, κ
Application
WB, IP, IF

Basic Information

Immunogen
Biotin conjugated to keyhole limpet haemocyanin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Biotin
Introduction
Biotin is a water-soluble B-vitamin that helps your body convert food into energy. It is especially important during pregnancy and breastfeeding. In addition, biotin is important for the health of your hair, skin and nails.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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