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Mouse Anti-BRCC3 Recombinant Antibody (CBYY-0795) (CBMAB-0798-YY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-0795
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, E, FC

Basic Information

Immunogen
Recombinant protein corresponding to amino acids 1-316 of BRCC3 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BRCA1/BRCA2-Containing Complex Subunit 3
Introduction
This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.
Entrez Gene ID
79184
UniProt ID
P46736
Alternative Names
BRCA1/BRCA2-Containing Complex Subunit 3; BRCA1/BRCA2-Containing Complex Subunit 36; BRCA1-A Complex Subunit BRCC36; BRISC Complex Subunit BRCC36; CXorf53; BRCC36; C6.1A;
Function
Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097).
Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690).
Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665).
Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193).
The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665).
Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097).
Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).
Biological Process
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Double-strand break repair Source: UniProtKB
Double-strand break repair via nonhomologous end joining Source: Reactome
Histone H2A K63-linked deubiquitination Source: UniProtKB
Positive regulation of DNA repair Source: UniProtKB
Protein deubiquitination Source: Reactome
Protein K63-linked deubiquitination Source: UniProtKB
Response to ionizing radiation Source: UniProtKB
Response to X-ray Source: MGI
Signal transduction involved in G2 DNA damage checkpoint Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus; Spindle pole. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:20656690, PubMed:26344097). Interaction with ABRAXAS2 retains BRCC3 in the cytoplasm (PubMed:20656690).
Involvement in disease
A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA.
More Infomation

Huang, X., Gan, H., Tan, J., Wang, T., Zhao, J., & Zhao, Y. (2021). BRCC3 promotes activation of the NLRP6 inflammasome following cerebral ischemia/reperfusion (I/R) injury in rats. Neuroscience Letters, 756, 135954.

Zhang, W., Tao, S. S., Wang, T., Zhang, J., Liu, X., Li, Y. T., ... & Yin, R. H. (2021). ABRO1 stabilizes the deubiquitinase BRCC3 through inhibiting its degradation mediated by the E3 ubiquitin ligase WWP2. FEBS letters, 595(2), 169-182.

Zhang, C., Qin, K., Han, Y., Xu, S., & Shao, X. (2021). Identification and Verification of Cdk5 Phosphorylated Deubiquitinating Enzyme BRCC3.

Cheng, X., Xu, S., Zhang, C., Qin, K., Yan, J., & Shao, X. (2020). The BRCC3 regulated by Cdk5 promotes the activation of neuronal NLRP3 inflammasome in Parkinson’s disease models. Biochemical and biophysical research communications, 522(3), 647-654.

Meyer, T., Jahn, N., Lindner, S., Röhner, L., Dolnik, A., Weber, D., ... & Krönke, J. (2020). Functional characterization of BRCC3 mutations in acute myeloid leukemia with t (8; 21)(q22; q22. 1). Leukemia, 34(2), 404-415.

Wang, C. Y., Deneen, B., & Tzeng, S. F. (2019). BRCA1/BRCA2‐containing complex subunit 3 controls oligodendrocyte differentiation by dynamically regulating lysine 63‐linked ubiquitination. Glia, 67(9), 1775-1792.

Hu, J., Wu, H., Wang, D., Yang, Z., & Dong, J. (2019). LncRNA ANRIL promotes NLRP3 inflammasome activation in uric acid nephropathy through miR-122-5p/BRCC3 axis. Biochimie, 157, 102-110.

Zhang, F., & Zhou, Q. (2018). Knockdown of BRCC3 exerts an anti‑tumor effect on cervical cancer in vitro. Molecular medicine reports, 18(6), 4886-4894.

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For research use only. Not intended for any clinical use.

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