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Mouse Anti-BUD23 Recombinant Antibody (2E12) (CBMAB-A9910-LY)

The product is antibody recognizes WBSCR22. The antibody 2E12 immunoassay techniques such as: WB, ELISA.
See all BUD23 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2E12
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
WBSCR22 (NP_059998.2, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BUD23, rRNA methyltransferase and ribosome maturation factor
Introduction
This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. [provided by RefSeq]
Entrez Gene ID
114049
UniProt ID
O43709
Alternative Names
HASJ4442; HUSSY-3; MGC19709; MGC2022; MGC5140; PP3381; WBMT
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N7 position of a guanine in 18S rRNA (PubMed:25851604).
Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity (PubMed:25851604).
Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed:25851604).
Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity (PubMed:24086612).
Locus-specific steroid receptor coactivator. Potentiates transactivation by glucocorticoid (NR3C1), mineralocorticoid (NR3C2), androgen (AR) and progesterone (PGR) receptors (PubMed:24488492).
Required for the maintenance of open chromatin at the TSC22D3/GILZ locus to facilitate NR3C1 loading on the response elements (PubMed:24488492).
Required for maintenance of dimethylation on histone H3 'Lys-79' (H3K79me2), although direct histone methyltransferase activity is not observed in vitro (PubMed:24488492).
Biological Process
Chromatin organization Source: UniProtKB-KW
Positive regulation of rRNA processing Source: UniProtKB
rRNA (guanine-N7)-methylation Source: UniProtKB
rRNA methylation Source: Reactome
Cellular Location
Cytoplasm; Nucleus; Nucleoplasm; Perinuclear region. Localized diffusely throughout the nucleus and the cytoplasm (PubMed:24488492). Localizes to a polarized perinuclear structure, overlapping partially with the Golgi and lysosomes (PubMed:25851604). Localization is not affected by glucocorticoid treatment (PubMed:24488492).
Involvement in disease
BUD23 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BUD23 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
PTM
May be ubiquitinated and targeted to degradation in response to proinflammatory cytokine signaling.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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