Mouse Anti-BUD23 Recombinant Antibody (2E12) (CBMAB-A9910-LY)

Name: Mouse Anti-BUD23 Recombinant Antibody (2E12)
SKU: CBMAB-A9910-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2E12

Application

WB, E, IP. IF

Immunogen

WBSCR22 (NP_059998.2, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

BUD23, rRNA methyltransferase and ribosome maturation factor

Introduction

This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. [provided by RefSeq]

Entrez Gene ID

114049

UniProt ID

O43709

Alternative Names

HASJ4442; HUSSY-3; MGC19709; MGC2022; MGC5140; PP3381; WBMT

Function

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N7 position of a guanine in 18S rRNA (PubMed:25851604).
Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity (PubMed:25851604).
Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed:25851604).
Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity (PubMed:24086612).
Locus-specific steroid receptor coactivator. Potentiates transactivation by glucocorticoid (NR3C1), mineralocorticoid (NR3C2), androgen (AR) and progesterone (PGR) receptors (PubMed:24488492).
Required for the maintenance of open chromatin at the TSC22D3/GILZ locus to facilitate NR3C1 loading on the response elements (PubMed:24488492).
Required for maintenance of dimethylation on histone H3 'Lys-79' (H3K79me2), although direct histone methyltransferase activity is not observed in vitro (PubMed:24488492).

Biological Process

Chromatin organization Source: UniProtKB-KW
Positive regulation of rRNA processing Source: UniProtKB
rRNA (guanine-N7)-methylation Source: UniProtKB
rRNA methylation Source: Reactome

Cellular Location

Cytoplasm; Nucleus; Nucleoplasm; Perinuclear region. Localized diffusely throughout the nucleus and the cytoplasm (PubMed:24488492). Localizes to a polarized perinuclear structure, overlapping partially with the Golgi and lysosomes (PubMed:25851604). Localization is not affected by glucocorticoid treatment (PubMed:24488492).

Involvement in disease

BUD23 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BUD23 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

PTM

May be ubiquitinated and targeted to degradation in response to proinflammatory cytokine signaling.

Black, J. J., Sardana, R., Elmir, E. W., & Johnson, A. W. (2020). Bud23 promotes the final disassembly of the small subunit Processome in Saccharomyces cerevisiae. PLoS genetics, 16(12), e1009215.

Black, J. J., Sardana, R., Elmir, E. W., & Johnson, A. W. (2020). Bud23 promotes the progression of the Small Subunit Processome to the pre-40S ribosome in Saccharomyces cerevisiae. bioRxiv.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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