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Rat Anti-CD3D Recombinant Antibody (CBYY-C1354) (CBMAB-C2792-YY)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYY-C1354
Antibody Isotype
IgG2a
Application
FC, IHC

Basic Information

Immunogen
CBAT6 thymocytes.
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC1:100
IHC1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD3d Molecule
Entrez Gene ID
915
UniProt ID
P04234
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).

In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).
Biological Process
Adaptive immune response Source: UniProtKB-KW
Cell surface receptor signaling pathway Source: GO_Central
Membrane organization Source: Reactome
Positive thymic T cell selection Source: UniProtKB
Regulation of immune response Source: Reactome
T cell receptor signaling pathway Source: Reactome
Cellular Location
Cell membrane
Involvement in disease
Immunodeficiency 19 (IMD19): An autosomal recessive form of severe combined immunodeficiency characterized by onset in early infancy of recurrent bacterial, viral, and fungal infections. Patients usually have chronic diarrhea, recurrent respiratory infections, and failure to thrive. Immunologic work-up shows a T-cell negative, B-cell positive, NK-cell positive phenotype.
Topology
Extracellular: 22-105
Helical: 106-126
Cytoplasmic: 127-171
PTM
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
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For research use only. Not intended for any clinical use.

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