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Mouse Anti-CD3G Recombinant Antibody (CBYJT-1100) (CBMAB-T0110-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-1100
Antibody Isotype
IgG1, κ
Application
ELISA, WB, IP, IF

Basic Information

Immunogen
Amino acids 21-100 mapping within an extracellular domain of CD3-γ of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD3g molecule, gamma (CD3-TCR complex)
Introduction
CD3G (CD3g Molecule) is a Protein Coding gene. Diseases related to CD3G include Immunodeficiency 17 and Enteropathy-Associated T-Cell Lymphoma. Among its related pathways are G-protein signaling N-RAS regulation pathway and Role of phospholipids in phagocytosis. Gene Ontology (GO) annotations related to CD3G include protein heterodimerization activity and receptor signaling complex scaffold activity. An important paralog of CD3G is CD3D.
Entrez Gene ID
917
UniProt ID
P09693
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).
In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769).
Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.
Biological Process
Adaptive immune response Source: UniProtKB-KW
Cell surface receptor signaling pathway Source: UniProtKB
Establishment or maintenance of cell polarity Source: UniProtKB
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Membrane organization Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptide cross-linking Source: Reactome
Positive thymic T cell selection Source: GO_Central
Protein-containing complex assembly Source: UniProtKB
Protein transport Source: UniProtKB
Regulation of immune response Source: Reactome
Regulation of lymphocyte apoptotic process Source: UniProtKB
T cell activation Source: UniProtKB
T cell receptor signaling pathway Source: Reactome
Cellular Location
Cell membrane
Involvement in disease
Immunodeficiency 17 (IMD17): An autosomal recessive primary immunodeficiency characterized by highly variable clinical severity. Some patients have onset of severe recurrent infections in early infancy that may be lethal, whereas others may be only mildly affected or essentially asymptomatic into young adulthood. More severely affected patients may have evidence of autoimmune disease or enteropathy. The immunologic pattern is similar among patients, showing partial T-cell lymphopenia, decreased amounts of the CD3 complex, and impaired proliferative responses to T-cell receptor dependent stimuli. The phenotype in some patients is reminiscent of severe combined immunodeficiency.
Topology
Extracellular: 23-116
Helical: 117-137
Cytoplasmic: 138-182
PTM
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8 (PubMed:2470098). Phosphorylated also by PKC; leading to the TCR complex down-regulation (PubMed:8187769).
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
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For research use only. Not intended for any clinical use.

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