Mouse Anti-CD3G Recombinant Antibody (CBYJT-1100) (CBMAB-T0110-YJ)

Name: Mouse Anti-CD3G Recombinant Antibody (CBYJT-1100)
SKU: CBMAB-T0110-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJT-1100

Application

ELISA, WB, IP, IF

Immunogen

Amino acids 21-100 mapping within an extracellular domain of CD3-γ of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CD3g molecule, gamma (CD3-TCR complex)

Introduction

CD3G (CD3g Molecule) is a Protein Coding gene. Diseases related to CD3G include Immunodeficiency 17 and Enteropathy-Associated T-Cell Lymphoma. Among its related pathways are G-protein signaling N-RAS regulation pathway and Role of phospholipids in phagocytosis. Gene Ontology (GO) annotations related to CD3G include protein heterodimerization activity and receptor signaling complex scaffold activity. An important paralog of CD3G is CD3D.

Entrez Gene ID

917

UniProt ID

P09693

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).
In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769).
Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Cell surface receptor signaling pathway Source: UniProtKB
Establishment or maintenance of cell polarity Source: UniProtKB
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Membrane organization Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptide cross-linking Source: Reactome
Positive thymic T cell selection Source: GO_Central
Protein-containing complex assembly Source: UniProtKB
Protein transport Source: UniProtKB
Regulation of immune response Source: Reactome
Regulation of lymphocyte apoptotic process Source: UniProtKB
T cell activation Source: UniProtKB
T cell receptor signaling pathway Source: Reactome

Cellular Location

Cell membrane

Involvement in disease

Immunodeficiency 17 (IMD17): An autosomal recessive primary immunodeficiency characterized by highly variable clinical severity. Some patients have onset of severe recurrent infections in early infancy that may be lethal, whereas others may be only mildly affected or essentially asymptomatic into young adulthood. More severely affected patients may have evidence of autoimmune disease or enteropathy. The immunologic pattern is similar among patients, showing partial T-cell lymphopenia, decreased amounts of the CD3 complex, and impaired proliferative responses to T-cell receptor dependent stimuli. The phenotype in some patients is reminiscent of severe combined immunodeficiency.

Topology

Extracellular: 23-116
Helical: 117-137
Cytoplasmic: 138-182

PTM

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8 (PubMed:2470098). Phosphorylated also by PKC; leading to the TCR complex down-regulation (PubMed:8187769).
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Garcillán, B., Fuentes, P., Marin, A. V., Megino, R. F., Chacon-Arguedas, D., Mazariegos, M. S., ... & Regueiro, J. R. (2021). CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex. Frontiers in Cell and Developmental Biology, 9, 1547.

Mario-Vásquez, J. E., Naranjo-González, C. A., Montiel, J., Zuluaga, L. M., Vásquez, A. M., Tobón-Castaño, A., ... & Segura, C. (2021). Association of variants in IL1B, TLR9, TREM1, IL10RA, and CD3G and Native American ancestry on malaria susceptibility in Colombian populations. Infection, Genetics and Evolution, 87, 104675.

Lee, W. I., Fan, W. L., Lu, C. H., Chen, S. H., Kuo, M. L., Lin, S. J., ... & Huang, J. L. (2019). A Novel CD3G Mutation in a Taiwanese Patient With Normal T Regulatory Function Presenting With the CVID Phenotype Free of Autoimmunity—Analysis of all Genotypes and Phenotypes. Frontiers in immunology, 10, 2833.

Rowe, J. H., Delmonte, O. M., Keles, S., Stadinski, B. D., Dobbs, A. K., Henderson, L. A., ... & Notarangelo, L. D. (2018). Patients with CD3G mutations reveal a role for human CD3γ in Treg diversity and suppressive function. Blood, The Journal of the American Society of Hematology, 131(21), 2335-2344.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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