Mouse Anti-CEP250 Recombinant Antibody (CBXC-0375) (CBMAB-C5707-CQ)

Name: Mouse Anti-CEP250 Recombinant Antibody (CBXC-0375)
SKU: CBMAB-C5707-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CEP250 Recombinant Antibody (CBXC-0375) (CBMAB-C5707-CQ) in IF

HeLa cells were transfected with indicated plasmids. Immunofluorescence staining were performed to show the colocalization of CEP250, Nek2A, and PHF5A. ...View More

Song, C., Zhang, Y., Li, Y., Bie, J., Wang, Z., Yang, X., ... & Luo, J. (2023). The phosphorylation of PHF5A by TrkA-ERK1/2-ABL1 cascade regulates centrosome separation. Cell Death & Disease, 14(2), 98.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CEP250 Recombinant Antibody (CBXC-0375) (CBMAB-C5707-CQ) in Immunoblot

HeLa cells were synchronized by thymidine block and released at the indicated times. Whole-cell lysates were analyzed with immunoblotting. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CEP250 Recombinant Antibody (CBXC-0375) (CBMAB-C5707-CQ) in Immunoblot

Immunoblotting showing protein expression of NEK2, C-NAP1, and rootletin. α-Tubulin was used as a loading control. ...View More

Chu, Z., & Gruss, O. J. (2022). Mitotic maturation compensates for premature centrosome splitting and PCM loss in human cep135 knockout cells. Cells, 11(7), 1189.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-0375

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 107-286 mapping near the N-terminus of C-Nap1 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Centrosomal Protein 250

Introduction

This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Entrez Gene ID

11190

UniProt ID

Q9BV73

Alternative Names

Centrosomal Protein 250; Centrosomal Nek2-Associated Protein 1; 250 KDa Centrosomal Protein; Centrosomal Protein 2; C-NAP1; CNAP1; CEP2;

Function

May be involved in ciliogenesis (PubMed:28005958).
Probably plays an important role in centrosome cohesion during interphase.

Biological Process

Centriole-centriole cohesion Source: UniProtKB
Ciliary basal body-plasma membrane docking Source: Reactome
Cilium assembly Source: UniProtKB
Detection of light stimulus involved in visual perception Source: UniProtKB
G2/M transition of mitotic cell cycle Source: Reactome
Mitotic cell cycle Source: UniProtKB
Non-motile cilium assembly Source: GO_Central
Positive regulation of protein localization to centrosome Source: UniProtKB
Protein localization Source: UniProtKB
Protein localization to organelle Source: UniProtKB
Regulation of centriole-centriole cohesion Source: UniProtKB
Regulation of G2/M transition of mitotic cell cycle Source: Reactome

Cellular Location

Centriole; Centrosome; Cilium basal body; Perinuclear region; Photoreceptor outer segment; Photoreceptor inner segment. Component of the core centrosome. In interphase cells, it specifically associates with the proximal ends of both mother and daughter centrioles. Associates with the centrosome in interphase cells. In mitotic cells, it dissociates from the mitotic spindle poles. At the end of cell division, it reaccumulates at centrosomes.

Involvement in disease

Cone-rod dystrophy and hearing loss 2 (CRDHL2): An autosomal recessive disease defined by the association of progressive cone-rod dystrophy with sensorineural hearing loss. Cone-rod dystrophy is characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa, due to cone photoreceptors degenerating at a higher rate than rod photoreceptors.

PTM

Differentially phosphorylated during cell cycle. Phosphorylation may regulate association/dissociation from centrosome. During M phase of mitosis, C-terminal part is phosphorylated by NEK2, suggesting that it may trigger the dissociation from the mitotic centrosome. Dephosphorylated in vitro by the PP1 phosphatase.

Zeng, L., Fan, X., Wang, X., Deng, H., Zhang, X., Zhang, K., ... & Liu, Z. (2020). Involvement of NEK2 and its interaction with NDC80 and CEP250 in hepatocellular carcinoma. BMC medical genomics, 13(1), 1-14.

Kubota, D., Gocho, K., Kikuchi, S., Akeo, K., Miura, M., Yamaki, K., ... & Kameya, S. (2018). CEP250 mutations associated with mild cone-rod dystrophy and sensorineural hearing loss in a Japanese family. Ophthalmic genetics, 39(4), 500-507.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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