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Mouse Anti-CEP250 Recombinant Antibody (CBXC-0375) (CBMAB-C5707-CQ)

This product is a mouse antibody that recognizes CEP250. The antibody CBXC-0375 can be used for immunoassay techniques such as: ELISA, WB.
See all CEP250 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXC-0375
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Centrosomal Protein 250
Introduction
This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Entrez Gene ID
11190
UniProt ID
Q9BV73
Alternative Names
Centrosomal Protein 250; Centrosomal Nek2-Associated Protein 1; 250 KDa Centrosomal Protein; Centrosomal Protein 2; C-NAP1; CNAP1; CEP2;
Function
May be involved in ciliogenesis (PubMed:28005958).
Probably plays an important role in centrosome cohesion during interphase.
Biological Process
Centriole-centriole cohesion Source: UniProtKB
Ciliary basal body-plasma membrane docking Source: Reactome
Cilium assembly Source: UniProtKB
Detection of light stimulus involved in visual perception Source: UniProtKB
G2/M transition of mitotic cell cycle Source: Reactome
Mitotic cell cycle Source: UniProtKB
Non-motile cilium assembly Source: GO_Central
Positive regulation of protein localization to centrosome Source: UniProtKB
Protein localization Source: UniProtKB
Protein localization to organelle Source: UniProtKB
Regulation of centriole-centriole cohesion Source: UniProtKB
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
Cellular Location
Centriole; Centrosome; Cilium basal body; Perinuclear region; Photoreceptor outer segment; Photoreceptor inner segment. Component of the core centrosome. In interphase cells, it specifically associates with the proximal ends of both mother and daughter centrioles. Associates with the centrosome in interphase cells. In mitotic cells, it dissociates from the mitotic spindle poles. At the end of cell division, it reaccumulates at centrosomes.
Involvement in disease
Cone-rod dystrophy and hearing loss 2 (CRDHL2): An autosomal recessive disease defined by the association of progressive cone-rod dystrophy with sensorineural hearing loss. Cone-rod dystrophy is characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa, due to cone photoreceptors degenerating at a higher rate than rod photoreceptors.
PTM
Differentially phosphorylated during cell cycle. Phosphorylation may regulate association/dissociation from centrosome. During M phase of mitosis, C-terminal part is phosphorylated by NEK2, suggesting that it may trigger the dissociation from the mitotic centrosome. Dephosphorylated in vitro by the PP1 phosphatase.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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