Mouse Anti-CEP63 Recombinant Antibody (CBXC-2136) (CBMAB-C5619-CQ)

Name: Mouse Anti-CEP63 Recombinant Antibody (CBXC-2136)
SKU: CBMAB-C5619-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-2136

Application

WB, IHC

Immunogen

Full length human recombinant protein of human CEP63.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500
IHC	1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Centrosomal Protein 63

Introduction

This gene encodes a protein with six coiled-coil domains. The protein is localized to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. Several alternatively spliced transcript variants have been found, but their biological validity has not been determined.

Entrez Gene ID

80254

UniProt ID

Q96MT8

Alternative Names

Centrosomal Protein 63; Centrosomal Protein 63kDa; Centrosomal Protein Of 63 KDa; Centrosome Protein CEP63; SCKL6; Cep63;

Function

Required for normal spindle assembly. Plays a key role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication. Reported to be required for centrosomal recruitment of CEP152; however, this function has been questioned (PubMed:21983783, PubMed:26297806).
Also recruits CDK1 to centrosomes (PubMed:21406398).
Plays a role in DNA damage response. Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and delay in mitotic progression (PubMed:21406398).

Biological Process

Cell division Source: UniProtKB-KW
Centriole replication Source: UniProtKB
Ciliary basal body-plasma membrane docking Source: Reactome
De novo centriole assembly involved in multi-ciliated epithelial cell differentiation Source: GO_Central
DNA damage checkpoint Source: UniProtKB
G2/M transition of mitotic cell cycle Source: Reactome
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
Signal transduction in response to DNA damage Source: UniProtKB
Spindle assembly Source: UniProtKB

Cellular Location

Centrosome; Centriole; Centriolar satellite. Colocalizes with CDK5RAP2, CEP152 and WDR62 in a discrete ring around the proximal end of the parental centriole. At this site, a cohesive structure is predicted to engage parental centrioles and procentrioles.

Involvement in disease

Seckel syndrome 6 (SCKL6): A rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.

Liu, C., Yu, F., Ma, R., Zhang, L., Du, G., Niu, D., & Yin, D. (2021). Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1. Oncology Reports, 46(3), 1-9.

Tischer, T., Yang, J., & Barford, D. (2021). The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly. Journal of Cell Science.

Mahjabeen, I., Maqsood, Y., Abbasi, R., Ahmed, M. W., & Kayani, M. A. (2021). Polymorphism in miRNA target sites of CEP-63 and CEP-152 ring complex influences expression of CEP genes and favors tumorigenesis in glioma. Future Oncology, (0).

Wei, Z., Kim, T. S., Ahn, J. I., Meng, L., Chen, Y., Ryu, E. K., ... & Lee, K. S. (2020). Requirement of the Cep57-Cep63 interaction for proper Cep152 recruitment and centriole duplication. Molecular and cellular biology, 40(10), e00535-19.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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