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Rabbit Anti-CLDN8 Recombinant Antibody (EG726) (V2LY-1206-LY66)


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Published Data
V2LY-1206-LY66-1
Rabbit Anti-CLDN8 Recombinant Antibody (EG726) (V2LY-1206-LY66) in IF
Infection of polarized Caco-2 epithelial cell monolayers by C. jejuni 81-176 results in the disruption of claudin-8 patterns. Epithelial cells were immunostained with α-claudin-8 (green) and α-C. jejuni (red) without infection (A), or after infection with C. jejuni wild-type (wt) strain (B), ΔhtrA knockout mutant (C), ΔhtrA complemented with protease inactive SA mutant (D) or wt htrA (E). Infection was performed for 12 h at an MOI of 100. DAPI staining (blue) was used for the nuclear DNA counterstaining. White arrows indicate claudin-8 redistribution to agglomerates. ...View More
Sharafutdinov, I., Esmaeili, D. S., Harrer, A., Tegtmeyer, N., Sticht, H., & Backert, S. (2020). Campylobacter jejuni serine protease HtrA cleaves the tight junction component claudin-8. Frontiers in Cellular and Infection Microbiology, 10, 590186.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY66-2
Rabbit Anti-CLDN8 Recombinant Antibody (EG726) (V2LY-1206-LY66) in IF
Infection of polarized T84 epithelial cell monolayers by C. jejuni 81-176 leads to disruption of claudin-8 and occludin in the tight junctions. Epithelial cells were immunostained with α-occludin (red), α-claudin-8 (green) and α-C. jejuni (magenta) without infection (A), or after infection with C. jejuni wild-type (wt) strain (B), ΔhtrA knockout mutant (C), protease inactive SA mutant (D) or ΔhtrA complemented with wt htrA (E). White arrows indicate occludin and claudin-8 redistribution to protein agglomerates. White boxes contain enlarged parts of tight junctions from corresponding smaller white boxes. ...View More
Sharafutdinov, I., Esmaeili, D. S., Harrer, A., Tegtmeyer, N., Sticht, H., & Backert, S. (2020). Campylobacter jejuni serine protease HtrA cleaves the tight junction component claudin-8. Frontiers in Cellular and Infection Microbiology, 10, 590186.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Rabbit
Specificity
Human
Clone
EG726
Antibody Isotype
IgG
Application
WB, IF

Basic Information

Immunogen
Peptide corresponding to amino acids 206-225 of human claudin-8.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
IF(ICC)1:100-1:1,000
WB1:500-1:5,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% sodium azide
Concentration
0.5 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Claudin 8
Entrez Gene ID
9073
UniProt ID
P56748
Function
Tight-junction protein required for paracellular chloride transport in the kidney. Mediates recruitment of CLDN4 to tight junction in the kidney. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Biological Process
Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Cellular Location
Cell membrane; Tight junction. Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.
Topology
Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-117
Helical: 118-138
Extracellular: 139-166
Helical: 167-187
Cytoplasmic: 188-225
PTM
Ubiquitinated by the BCR(KLHL3) E3 ubiquitin ligase complex in the kidney, leading to its degradation.
More Infomation

Zhang, Y., Zheng, A., Lu, H., Jin, Z., Peng, Z., & Jin, F. (2020). The expression and prognostic significance of claudin-8 and androgen receptor in breast cancer. OncoTargets and therapy, 13, 3437.

Li, M., Zhao, J., Cao, M., Liu, R., Chen, G., Li, S., ... & Gong, S. (2020). Mast cells-derived MiR-223 destroys intestinal barrier function by inhibition of CLDN8 expression in intestinal epithelial cells. Biological research, 53(1), 1-11.

Nattramilarasu, P. K., Bücker, R., Lobo de Sá, F. D., Fromm, A., Nagel, O., Lee, I. F. M., ... & Schulzke, J. D. (2020). Campylobacter concisus impairs sodium absorption in colonic epithelium via ENaC dysfunction and claudin-8 disruption. International journal of molecular sciences, 21(2), 373.

Zhou, S., Piao, X., Wang, C., Wang, R., & Song, Z. (2019). Identification of claudin‑1,‑3,‑7 and‑8 as prognostic markers in human laryngeal carcinoma. Molecular medicine reports, 20(1), 393-400.

Cheng, B., Rong, A., Zhou, Q., & Li, W. (2019). CLDN8 promotes colorectal cancer cell proliferation, migration, and invasion by activating MAPK/ERK signaling. Cancer management and research, 11, 3741.

Li, L., Huang, S., Wang, H., Chao, K., Ding, L., Feng, R., ... & Zhang, S. (2018). Cytokine IL9 triggers the pathogenesis of inflammatory bowel disease through the miR21-CLDN8 pathway. Inflammatory bowel diseases, 24(10), 2211-2223.

Nedjat-Shokouhi, B. (2018). Pathogenesis of Ulcerative Colitis: the role of Claudin-8 in epithelial barrier function and inflammation (Doctoral dissertation, UCL (University College London)).

Ashikari, D., Takayama, K. I., Obinata, D., Takahashi, S., & Inoue, S. (2017). CLDN 8, an androgen‐regulated gene, promotes prostate cancer cell proliferation and migration. Cancer science, 108(7), 1386-1393.

Qi, J., Yang, Y., Hao, P., & Xu, J. (2017). Transcription Factor SOX9 Promotes Osteosarcoma Cell Growth by Repressing Claudin-8 Expression [Retraction]. The Tohoku journal of experimental medicine, 241(1), 55-63.

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For research use only. Not intended for any clinical use.

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