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Mouse Anti-CNN3 Recombinant Antibody (C6732) (V2LY-1206-LY447)


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Published Data
V2LY-1206-LY447-1
Mouse Anti-CNN3 Recombinant Antibody (C6732) (V2LY-1206-LY447) in IF
Cnn3 and α-actinin-1 dynamics were measured by FRAP in contractile fibers. Representative GFP-α-actinin-1 and GFP-Cnn3 expressing cells used in the experiments are shown of the left, and fluorescence recoveries of each case shown on the right. White boxes represent regions of interest (ROI). Scale bars, 10 μm. ...View More
Ciuba, K., Hawkes, W., Tojkander, S., Kogan, K., Engel, U., Iskratsch, T., & Lappalainen, P. (2018). Calponin-3 is critical for coordinated contractility of actin stress fibers. Scientific reports, 8(1), 17670.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY447-2
Mouse Anti-CNN3 Recombinant Antibody (C6732) (V2LY-1206-LY447) in IF
Control, Cnn3 knockdown, and Cnn3 knockout U2OS cells transfected with GFP-actin were imaged for 30 min, and frames were captured every 30 s. Representative time-lapse frames for each condition are presented. Examples of stress fiber breakage events in Cnn3 knockdown and knockout cells are marked by yellow asterisks ('*'). Scale bars, 10 μm. Please, see supplemental videos 1-3 for better visualization of abnormal stress fiber dynamics and breakage events. ...View More
Ciuba, K., Hawkes, W., Tojkander, S., Kogan, K., Engel, U., Iskratsch, T., & Lappalainen, P. (2018). Calponin-3 is critical for coordinated contractility of actin stress fibers. Scientific reports, 8(1), 17670.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
C6732
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 275-329 mapping at the C-terminus of Calponin 3 of human and rat.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
<0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
calponin 3, acidic
Entrez Gene ID
Human1266
Mouse71994
Rat54321
UniProt ID
HumanQ15417
MouseQ9DAW9
RatP37397
Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Biological Process
Actomyosin structure organization Source: InterPro
Epithelial cell differentiation Source: UniProtKB
Negative regulation of ATPase activity Source: Ensembl
Cellular Location
Actin cytoskeleton; Cytosol; Adherens junction; Dendritic spine; Focal adhesion; Neuronal cell body; Postsynaptic density
More Infomation

Yang, C., Zhu, S., Feng, W., & Chen, X. (2021). Calponin 3 suppresses proliferation, migration and invasion of non‑small cell lung cancer cells. Oncology Letters, 22(2), 1-9.

She, Y., Li, C., Jiang, T., Lei, S., Zhou, S., Shi, H., & Chen, R. (2021). Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway. Frontiers in physiology, 12, 862.

Maddala, R., Mongan, M., Xia, Y., & Rao, P. V. (2020). Calponin-3 deficiency augments contractile activity, plasticity, fibrogenic response and Yap/Taz transcriptional activation in lens epithelial cells and explants. Scientific reports, 10(1), 1-15.

Dai, F., Luo, F., Zhou, R., Zhou, Q., Xu, J., Zhang, Z., ... & Song, L. (2020). Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma. Aging (Albany NY), 12(14), 14037.

Xia, L., Yue, Y., Li, M., Zhang, Y. N., Zhao, L., Lu, W., ... & Xie, X. (2020). CNN3 acts as a potential oncogene in cervical cancer by affecting RPLP1 mRNA expression. Scientific reports, 10(1), 1-10.

Ma, C., Gu, R., Wang, X., He, S., Bai, J., Zhang, L., ... & Zhu, D. (2020). circRNA CDR1as promotes pulmonary artery smooth muscle cell calcification by Upregulating CAMK2D and CNN3 via sponging miR-7-5p. Molecular Therapy-Nucleic Acids, 22, 530-541.

Nair, V. A., Al-Khayyal, N. A., Sivaperumal, S., & Abdel-Rahman, W. M. (2019). Calponin 3 promotes invasion and drug resistance of colon cancer cells. World Journal of Gastrointestinal Oncology, 11(11), 971.

Hong, K. S., Kim, H., Kim, S. H., Kim, M., & Yoo, J. (2019). Calponin 3 regulates cell invasion and doxorubicin resistance in gastric cancer. Gastroenterology Research and Practice, 2019.

Ciuba, K., Hawkes, W., Tojkander, S., Kogan, K., Engel, U., Iskratsch, T., & Lappalainen, P. (2018). Calponin-3 is critical for coordinated contractility of actin stress fibers. Scientific reports, 8(1), 1-11.

Abdel-Rahman, W. M., Nair, V., & Al-khayyal, N. (2018). PO-206 Calponin 3 (CNN3) promotes epithelial to mesenchymal transition and drug resistance of colon cancer cells. ESMO Open, 3, A100-A101.

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For research use only. Not intended for any clinical use.

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