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Mouse Anti-COX6C Recombinant Antibody (CBYY-C2066) (V2LY-1206-LY885)


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Published Data
V2LY-1206-LY885-1
Mouse Anti-COX6C Recombinant Antibody (CBYY-C2066) (V2LY-1206-LY885) in ELISA
Mitochondrial membrane proteins on the surface of EVs are unique biomarkers for cancer. (a,b) The levels of mitochondrial inner membrane proteins, MT-CO2 (a) and COX6c (b) were examined in plasma healthy and melanoma patients. (c) The sandwich ELISA system was validated with melanoma tissue-derived EVs and non-melanoma-derived EVs. Data are presented as the mean ± SD. (d-f) The levels of mitochondrial proteins (MT-CO2 and COX6c) were examined in blood plasma from melanoma patients (n = 21) (d), ovarian cancer patients (n = 62) (e), and breast cancer patients (n = 13) (f). Healthy control was six. Whiskers show the minimum-maximum and lines inside box represent the median. *p < 0.05, **p < 0.001. ...View More
Jang, S. C., Crescitelli, R., Cvjetkovic, A., Belgrano, V., Olofsson Bagge, R., Sundfeldt, K., ... & Lötvall, J. (2019). Mitochondrial protein enriched extracellular vesicles discovered in human melanoma tissues can be detected in patient plasma. Journal of extracellular vesicles, 8(1), 1635420.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C2066
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Full length of human COX6c.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome C Oxidase Subunit 6C
Entrez Gene ID
1345
UniProt ID
P09669
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Biological Process
Generation of precursor metabolites and energy Source: ProtInc
Mitochondrial electron transport, cytochrome c to oxygen Source: Reactome
Cellular Location
Mitochondrion inner membrane
Topology
Mitochondrial matrix: 1-13
Helical: 14-54
Mitochondrial intermembrane: 55-75
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For research use only. Not intended for any clinical use.

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