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Mouse Anti-CRLF1 Recombinant Antibody (C7374) (V2LY-1206-LY1192)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
C7374
Application
ELISA, IP, WB
Immunogen
Recombinant human CRLF1.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
cytokine receptor-like factor 1
Entrez Gene ID
9244
UniProt ID
O75462
Function
In complex with CLCF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (Probable). May also play a regulatory role in the immune system.
Biological Process
Cytokine-mediated signaling pathway Source: GO_Central
Interleukin-27-mediated signaling pathway Source: Reactome
Negative regulation of motor neuron apoptotic process Source: Ensembl
Negative regulation of neuron apoptotic process Source: BHF-UCL
Positive regulation of cell population proliferation Source: BHF-UCL
Positive regulation of tyrosine phosphorylation of STAT protein Source: BHF-UCL
Ureteric bud development Source: Ensembl
Cellular Location
Secreted
Involvement in disease
Crisponi/Cold-induced sweating syndrome 1 (CISS1):
An autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Patients manifest, in the neonatal period, orofacial weakness with impaired sucking and swallowing, resulting in poor feeding. Affected infants show a tendency to startle, with contractions of the facial muscles in response to tactile stimuli or during crying, trismus, abundant salivation, and opisthotonus. These features are referred to as Crisponi syndrome and can result in early death in infancy. Patients who survive into childhood have hyperhidrosis, mainly of the upper body, in response to cold temperatures, and sweat very little with heat. Additional abnormalities include a high-arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis.

Crisponi, L., Buers, I., & Rutsch, F. (2022). CRLF1 and CLCF1 in Development, Health and Disease. International Journal of Molecular Sciences, 23(2), 992.

Li, Y., Xun, J., Wang, B., Ma, Y., Zhang, L., Yang, L., ... & Zhang, Q. (2021). miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer. Journal of translational medicine, 19(1), 1-14.

Xu, H., Ding, C., Guo, C., Xiang, S., Wang, Y., Luo, B., & Xiang, H. (2021). Suppression of CRLF1 promotes the chondrogenic differentiation of bone marrow-derived mesenchymal stem and protects cartilage tissue from damage in osteoarthritis via activation of miR-320. Molecular Medicine, 27(1), 1-11.

Zheng, Z., Ao, X., Li, P., Lian, Z., Jiang, T., Zhang, Z., & Wang, L. (2020). CRLF1 is a key regulator in the ligamentum flavum hypertrophy. Frontiers in cell and developmental biology, 858.

Yu, S. T., Zhong, Q., Chen, R. H., Han, P., Li, S. B., Zhang, H., ... & Huang, X. M. (2018). CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways. Cell death & disease, 9(3), 1-13.

Zhong, Q., Yu, S., Huang, X., & Zeng, M. (2018). CRLF1 promotes proliferation and metastasis in papillary thyroid carcinoma via activating MAPK/ERK and PI3K/AKT pathway.

Busch, A., Žarković, M., Lowe, C., Jankofsky, M., Ganschow, R., Buers, I., ... & Hübner, C. A. (2017). Mutations in CRLF1 cause familial achalasia. Clinical genetics, 92(1), 104-108.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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