Mouse Anti-CRNKL1 Recombinant Antibody (C0664) (V2LY-1206-LY1207)

Name: Mouse Anti-CRNKL1 Recombinant Antibody (C0664)
SKU: V2LY-1206-LY1207
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

C0664

Application

WB, IP

Immunogen

Recombinant protein corresponding to the C-terminal region of human CRN.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Crooked Neck Pre-MRNA Splicing Factor 1

Entrez Gene ID

Human	51340
Mouse	66877
Rat	100910202

UniProt ID

Human	Q9BZJ0
Mouse	P63154
Rat	P63155

Function

Involved in pre-mRNA splicing process.

Biological Process

mRNA splicing, via spliceosome Source: UniProtKB
Spliceosomal complex assembly Source: UniProtKB

Cellular Location

Nucleus; Nucleus speckle. Colocalizes with core spliceosomal snRNP proteins (PubMed:12084575).

Xiao, H., Wyler, E., Milek, M., Grewe, B., Kirchner, P., Ekici, A., ... & Überla, K. (2021). CRNKL1 is a highly selective regulator of intron-retaining HIV-1 and cellular mRNAs. Mbio, 12(1), e02525-20.

Li, Z., Yao, Q., Zhao, S., Wang, Z., & Li, Y. (2017). Protein coding gene CRNKL1 as a potential prognostic biomarker in esophageal adenocarcinoma. Artificial Intelligence in Medicine, 76, 1-6.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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