Mouse Anti-CYB5R3 Recombinant Antibody (CBFYC-2554) (V2LY-0125-LY839)

Name: Mouse Anti-CYB5R3 Recombinant Antibody (CBFYC-2554)
SKU: V2LY-0125-LY839
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CYB5R3 Recombinant Antibody (CBFYC-2554) (V2LY-0125-LY839) in WB

Western blots of B5R (A), B5 (B), and CPR (C) obtained from scr-RNA and B5R, B5 and CPR siRNA-treated mouse aortic SMCs. The first three bands correspond to known amounts of purified B5R (A), B5 (B), or CPR (C). Bands 4 and 5 are from scr-RNA and B5R (A), B5 (B), or CPR (C) siRNA-treated cell homogenates, respectively. The bar graphs show the mean value ± SD for n = 3. From the ratio of each measured protein to total cellular protein concentration, the cellular concentrations of B5R, B5, and CPR are estimated at 0.88 μM, 0.38 μM, and 0.15 μM, respectively. ...View More

Ilangovan, G., Khaleel, S. A., Kundu, T., Hemann, C., El-Mahdy, M. A., & Zweier, J. L. (2021). Defining the reducing system of the NO dioxygenase cytoglobin in vascular smooth muscle cells and its critical role in regulating cellular NO decay. Journal of Biological Chemistry, 296. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CYB5R3 Recombinant Antibody (CBFYC-2554) (V2LY-0125-LY839) in Immunoblotting

Relative amount of b5R in rat aortic vascular SMCs was estimated by quantitative immunoblotting before and after knockdown of b5R. Positions of nearest molecular weight markers are shown. (a) NO consumption by 7 × 106 control SMCs per ml and by 7 × 106 b5R-KD SMCs per ml. (b) NO was injected into the solution to achieve an initial concentration of 0.5 μM. Means and standard errors of the rate of NO decay by 7 × 106 SMCs per ml and 7 × 106 b5R-KD SMCs per ml (c). ...View More

Liu, X., El-Mahdy, M. A., Boslett, J., Varadharaj, S., Hemann, C., Abdelghany, T. M., ... & Zweier, J. L. (2017). Cytoglobin regulates blood pressure and vascular tone through nitric oxide metabolism in the vascular wall. Nature communications, 8(1), 14807. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBFYC-2554

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 1-60 mapping at the N-terminus of human CYB5R3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cytochrome B5 Reductase 3

Entrez Gene ID

1727

UniProt ID

P00387

Function

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

Biological Process

Blood circulation Source: ProtInc
Cholesterol biosynthetic process Source: UniProtKB-KW
L-ascorbic acid metabolic process Source: Reactome
Neutrophil degranulation Source: Reactome
Nitric oxide biosynthetic process Source: FlyBase
Xenobiotic metabolic process Source: Reactome

Cellular Location

Isoform 1: Endoplasmic reticulum membrane; Mitochondrion outer membrane
Isoform 2: Cytoplasm. Produces the soluble form found in erythrocytes.

Involvement in disease

Methemoglobinemia CYB5R3-related (METHB-CYB5R3):
A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well-tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms.

More Infomation

References

Deorukhkar, A., Kulkarni, A., & Kedar, P. (2022). Three novel mutations in CYB5R3 gene causing NADH-cytochrome b5 reductase enzyme deficiency leads to recessive congenital methaemoglobinemia. Molecular Biology Reports, 1-7.

Wood, K. C., Durgin, B. G., Schmidt, H. M., Hahn, S. A., Baust, J. J., Bachman, T., ... & Straub, A. C. (2019). Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice. Blood advances, 3(23), 4104-4116.

Carew, N. T., Altmann, H. M., Galley, J. C., Hahn, S., Miller, M. P., Shiva, S., ... & Straub, A. C. (2018). Cytochrome b5 Reductase 3 Regulates Myoglobin Redox State and Controls Cardiac Function. Circulation Research, 123(Suppl_1), A103-A103.

Kedar, P. S., Gupta, V., Warang, P., Chiddarwar, A., & Madkaikar, M. (2018). Novel mutation (R192C) in CYB5R3 gene causing NADH-cytochrome b5 reductase deficiency in eight Indian patients associated with autosomal recessive congenital methemoglobinemia type-I. Hematology, 23(8), 567-573.

Carew, N. T., Altmann, H. M., Galley, J. C., Hahn, S., Miller, M. P., Shiva, S., ... & Straub, A. C. (2017). Cytochrome b5 reductase 3 is essential for cardiomyocyte function. Circulation, 136(suppl_1), A20733-A20733.

Rahaman, M. M., Nguyen, A. T., Miller, M. P., Hahn, S. A., Sparacino-Watkins, C., Jobbagy, S., ... & Straub, A. C. (2017). Cytochrome b5 reductase 3 modulates soluble guanylate cyclase redox state and cGMP signaling. Circulation research, 121(2), 137-148.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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Second antibody and isotype control

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