Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CYP46A1 Recombinant Antibody (CBXC-3156) (V2LY-0125-LY1001)


See all CYP46A1 antibodies
Online Inquiry
Published Data
V2LY-0125-LY1001-1
Mouse Anti-CYP46A1 Recombinant Antibody (CBXC-3156) (V2LY-0125-LY1001) in WB
Brain CYP46 expression levels in wt and AD mice orally administered with SLAB51 for 4 and 12 months. Representative immunoblots and corresponding densitometric analyses derived from six separate blots are shown. Equal protein loading was verified by using an anti-GAPDH antibody. ...View More
Bonfili, L., Cuccioloni, M., Gong, C., Cecarini, V., Spina, M., Zheng, Y., ... & Eleuteri, A. M. (2022). Gut microbiota modulation in Alzheimer's disease: Focus on lipid metabolism. Clinical Nutrition, 41(3), 698-708. Distributed under Open Access license CC BY 4.0, without modification.
V2LY-0125-LY1001-2
Mouse Anti-CYP46A1 Recombinant Antibody (CBXC-3156) (V2LY-0125-LY1001) in WB
Western blot images showing the expression of ABCA1, MMP9, AQP4, and CYP46 proteins in different groups. ...View More
Li, Z., Li, J. N., Li, Q., Liu, C., Zhou, L. H., Zhang, Q., & Xu, Y. (2022). Cholesterol efflux regulator ABCA1 exerts protective role against high shear stress-induced injury of HBMECs via regulating PI3K/Akt/eNOS signaling. BMC neuroscience, 23(1), 61. Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXC-3156
Antibody Isotype
IgG1, κ
Application
WB, IF

Basic Information

Immunogen
Recombinant human CYP46.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
cytochrome P450, family 46, subfamily A, polypeptide 1
Entrez Gene ID
10858
UniProt ID
Q9Y6A2
Function
P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation (PubMed:10377398, PubMed:14640697, PubMed:25017465, PubMed:18621681).

By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning (By similarity).

Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis (PubMed:12077124, PubMed:14640697, PubMed:28190002).

Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain (PubMed:25017465).

May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole (PubMed:20667828).
Biological Process
Bile acid biosynthetic process Source: Reactome
Cholesterol catabolic process Source: UniProtKB
Nervous system development Source: ProtInc
Progesterone metabolic process Source: UniProtKB
Protein localization to membrane raft Source: ARUK-UCL
Regulation of long-term synaptic potentiation Source: UniProtKB
Sterol metabolic process Source: Reactome
Xenobiotic metabolic process Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane; Postsynapse; Presynapse; Dendrite
Topology
Helical: 3-23
More Infomation

El‐Darzi, N., Mast, N., Petrov, A. M., & Pikuleva, I. A. (2021). 2‐Hydroxypropyl‐β‐cyclodextrin reduces retinal cholesterol in wild‐type and Cyp27a1−/− Cyp46a1−/− mice with deficiency in the oxysterol production. British journal of pharmacology, 178(16), 3220-3234.

Ali, T., Hannaoui, S., Nemani, S., Tahir, W., Zemlyankina, I., Cherry, P., ... & Gilch, S. (2021). Oral administration of repurposed drug targeting Cyp46A1 increases survival times of prion infected mice. Acta neuropathologica communications, 9(1), 1-14.

Han, M., Wang, S., Yang, N., Wang, X., Zhao, W., Saed, H. S., ... & Wang, J. (2020). Therapeutic implications of altered cholesterol homeostasis mediated by loss of CYP46A1 in human glioblastoma. EMBO molecular medicine, 12(1), e10924.

Petrov, A. M., Lam, M., Mast, N., Moon, J., Li, Y., Maxfield, E., & Pikuleva, I. A. (2019). CYP46A1 activation by efavirenz leads to behavioral improvement without significant changes in amyloid plaque load in the brain of 5XFAD mice. Neurotherapeutics, 16(3), 710-724.

Petrov, A. M., & Pikuleva, I. A. (2019). Cholesterol 24-hydroxylation by CYP46A1: benefits of modulation for brain diseases. Neurotherapeutics, 16(3), 635-648.

Kacher, R., Lamazière, A., Heck, N., Kappes, V., Mounier, C., Despres, G., ... & Betuing, S. (2019). CYP46A1 gene therapy deciphers the role of brain cholesterol metabolism in Huntington’s disease. Brain, 142(8), 2432-2450.

Theofilopoulos, S., de Oliveira, W. A. A., Yang, S., Yutuc, E., Saeed, A., Abdel-Khalik, J., ... & Arenas, E. (2019). 24 (S), 25-Epoxycholesterol and cholesterol 24S-hydroxylase (CYP46A1) overexpression promote midbrain dopaminergic neurogenesis in vivo. Journal of Biological Chemistry, 294(11), 4169-4176.

Mast, N., Verwilst, P., Wilkey, C. J., Guengerich, F. P., & Pikuleva, I. A. (2019). In vitro activation of cytochrome P450 46A1 (CYP46A1) by efavirenz-related compounds. Journal of medicinal chemistry, 63(12), 6477-6488.

Mast, N., Anderson, K. W., Johnson, K. M., Phan, T. T., Guengerich, F. P., & Pikuleva, I. A. (2017). In vitro cytochrome P450 46A1 (CYP46A1) activation by neuroactive compounds. Journal of Biological Chemistry, 292(31), 12934-12946.

Mast, N., Lin, J. B., Anderson, K. W., Bjorkhem, I., & Pikuleva, I. A. (2017). Transcriptional and post-translational changes in the brain of mice deficient in cholesterol removal mediated by cytochrome P450 46A1 (CYP46A1). PLoS One, 12(10), e0187168.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CYP46A1 Recombinant Antibody (CBXC-3156)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry