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Rabbit Anti-DFFA Recombinant Antibody (EG949) (CBMAB-EN1128-LY)

The product is antibody recognizes DFFA. The antibody EG949 immunoassay techniques such as: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000.
See all DFFA antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG949
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human DFF-45.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DNA Fragmentation Factor Subunit Alpha
Introduction
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human1676
Mouse13347
Rat114214
UniProt ID
HumanO00273
MouseO54786
RatA0A0G2JY18
Alternative Names
DNA Fragmentation Factor Subunit Alpha; DNA Fragmentation Factor, 45kDa, Alpha Polypeptide; DNA Fragmentation Factor 45 KDa Subunit; Inhibitor Of CAD; DFF-45; DFF45;
Function
Inhibitor of the caspase-activated DNase (DFF40).
Biological Process
Apoptotic DNA fragmentation Source: Reactome
Chaperone-mediated protein folding Source: CAFA
Negative regulation of apoptotic DNA fragmentation Source: MGI
Negative regulation of deoxyribonuclease activity Source: CAFA
Negative regulation of execution phase of apoptosis Source: BHF-UCL
Positive regulation of apoptotic process Source: Ensembl
Regulation of apoptotic process Source: GO_Central
Thymocyte apoptotic process Source: Ensembl
Cellular Location
Cytoplasm
PTM
Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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