Mouse Anti-DFFA Recombinant Antibody (CBYY-I1006) (CBMAB-I2174-YY)

Name: Mouse Anti-DFFA Recombinant Antibody (CBYY-I1006)
SKU: CBMAB-I2174-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-I1006

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 199-226 near the C-terminus of human ICAD.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

DNA Fragmentation Factor Subunit Alpha

Introduction

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Entrez Gene ID

Human	1676
Mouse	13347
Rat	114214

UniProt ID

Human	O00273
Mouse	O54786
Rat	A0A0G2JY18

Alternative Names

DNA Fragmentation Factor Subunit Alpha; DNA Fragmentation Factor, 45kDa, Alpha Polypeptide; DNA Fragmentation Factor 45 KDa Subunit; Inhibitor Of CAD; DFF-45; DFF45;

Function

Inhibitor of the caspase-activated DNase (DFF40).

Biological Process

Apoptotic DNA fragmentation Source: Reactome
Chaperone-mediated protein folding Source: CAFA
Negative regulation of apoptotic DNA fragmentation Source: MGI
Negative regulation of deoxyribonuclease activity Source: CAFA
Negative regulation of execution phase of apoptosis Source: BHF-UCL
Positive regulation of apoptotic process Source: Ensembl
Regulation of apoptotic process Source: GO_Central
Thymocyte apoptotic process Source: Ensembl

Cellular Location

Cytoplasm

PTM

Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.

Fawzy, M. S., Toraih, E. A., Ibrahiem, A., Abdeldayem, H., Mohamed, A. O., & Abdel-Daim, M. M. (2017). Evaluation of miRNA-196a2 and apoptosis-related target genes: ANXA1, DFFA and PDCD4 expression in gastrointestinal cancer patients: A pilot study. PLoS One, 12(11), e0187310.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

DFFA Matched Antibody Pair (335) (CAT#: APMAB-335LY)

DFFA Matched Antibody Pair (336) (CAT#: APMAB-336LY)

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Mouse Anti-DFFA Recombinant Antibody (D0749) (CAT#: CBMAB-D0749-YC)

DFFA Matched Antibody Pair (336) (CAT#: APMAB-336LY)

Rabbit Anti-DFFA Recombinant Antibody (EG949) (CAT#: V2LY-0425-LY220)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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