Mouse Anti-DFFA Recombinant Antibody (CBT3777) (V2LY-0625-LY1321)

Name: Mouse Anti-DFFA Recombinant Antibody (CBT3777)
SKU: V2LY-0625-LY1321
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3777

Application

Immunogen

Purified recombinant fragment of human DFFA (AA: 179-331) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DNA Fragmentation Factor Subunit Alpha

Entrez Gene ID

1676

UniProt ID

O00273

Function

Inhibitor of the caspase-activated DNase (DFF40).

Biological Process

Apoptotic DNA fragmentation Source: Reactome
Chaperone-mediated protein folding Source: CAFA
Negative regulation of apoptotic DNA fragmentation Source: MGI
Negative regulation of deoxyribonuclease activity Source: CAFA
Negative regulation of execution phase of apoptosis Source: BHF-UCL
Positive regulation of apoptotic process Source: Ensembl
Regulation of apoptotic process Source: GO_Central
Thymocyte apoptotic process Source: Ensembl

Cellular Location

Cytoplasm

PTM

Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.

Fawzy, M. S., Toraih, E. A., Ibrahiem, A., Abdeldayem, H., Mohamed, A. O., & Abdel-Daim, M. M. (2017). Evaluation of miRNA-196a2 and apoptosis-related target genes: ANXA1, DFFA and PDCD4 expression in gastrointestinal cancer patients: A pilot study. PLoS One, 12(11), e0187310.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

DFFA Matched Antibody Pair (335) (CAT#: APMAB-335LY)

DFFA Matched Antibody Pair (336) (CAT#: APMAB-336LY)

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Rabbit Anti-DFFA Recombinant Antibody (EG949) (CAT#: V2LY-0425-LY220)

Mouse Anti-DFFA Recombinant Antibody (D0743) (CAT#: CBMAB-D0743-YC)

DFFA Matched Antibody Pair (336) (CAT#: APMAB-336LY)

Mouse Anti-DFFA Recombinant Antibody (D0748) (CAT#: CBMAB-D0748-YC)

Mouse Anti-DFFA Recombinant Antibody (D0749) (CAT#: CBMAB-D0749-YC)

DFFA Matched Antibody Pair (335) (CAT#: APMAB-335LY)

Mouse Anti-DFFA Recombinant Antibody (CBYY-I1006) (CAT#: CBMAB-I2174-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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