Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-DHCR24 Recombinant Antibody (CBXS-1259) (V2LY-0425-LY259)


See all DHCR24 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXS-1259
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 1-300 mapping at the N-terminus of human DHCR24.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
24-Dehydrocholesterol Reductase
Entrez Gene ID
Human1718
Mouse74754
UniProt ID
HumanQ15392
MouseQ8VCH6
Function
Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis (PubMed:11519011, PubMed:21671375, PubMed:25637936, PubMed:22178193).

In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress (PubMed:11007892, PubMed:22010141).

Also protects against amyloid-beta peptide-induced apoptosis (PubMed:11007892).
Biological Process
Apoptotic process Source: UniProtKB
Cell cycle arrest Source: UniProtKB
Cholesterol biosynthetic process Source: MGI
Cholesterol biosynthetic process via desmosterol Source: UniProtKB
Cholesterol biosynthetic process via lathosterol Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Regulation of neuron death Source: UniProtKB
Response to oxidative stress Source: UniProtKB
Skin development Source: UniProtKB
Steroid metabolic process Source: GO_Central
Tissue development Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Golgi apparatus membrane
Involvement in disease
Desmosterolosis (DESMOS):
Rare autosomal recessive disorder characterized by multiple congenital anomalies and elevated levels of the cholesterol precursor desmosterol in plasma, tissue, and cultured cells.
Topology
Lumenal: 23-31
Helical: 32-52
Cytoplasmic: 53-516
More Infomation

Yao, M., Li, F., Xu, L., Ma, L., & Zhang, S. (2022). 24-Dehydrocholesterol Reductase alleviates oxidative damage-induced apoptosis in alveolar epithelial cells via regulating Phosphatidylinositol-3-Kinase/Protein Kinase B activation. Bioengineered, 13(1), 155-163.

Kohlhaas, J., Jäger, M. A., Lust, L., De La Torre, C., Hecker, M., & Korff, T. (2021). Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression. Experimental Cell Research, 399(2), 112446.

Mack, Y. S. I., Dehari, M., Morooka, N., & Nagata, S. (2021). Identification and Characterization of 24-Dehydrocholesterol Reductase (DHCR24) in the Two-Spotted Cricket, Gryllus bimaculatus. Insects, 12(9), 782.

Yavuz, U., Alaylıoğlu, M., Şengül, B., Karras, S. N., Gezen-Ak, D., & Dursun, E. (2021). Protein disulfide isomerase A3 might be involved in the regulation of 24-dehydrocholesterol reductase via vitamin D equilibrium in primary cortical neurons. In Vitro Cellular & Developmental Biology-Animal, 57(7), 704-714.

Wang, N., Gong, Z., Wang, J., Xu, W., Yang, Q., & Chen, S. (2021). Characterization of Chinese tongue sole (Cynoglossus semilaevis) 24-dehydrocholesterol reductase: Expression profile, epigenetic modification, and its knock-down effect. General and comparative endocrinology, 312, 113870.

Qiu, T., Cao, J., Chen, W., Wang, J., Wang, Y., Zhao, L., ... & Gu, H. (2020). 24‐Dehydrocholesterol reductase promotes the growth of breast cancer stem‐like cells through the Hedgehog pathway. Cancer science, 111(10), 3653-3664.

Tallorin, L., Villareal, V. A., Hsia, C. Y., Rodgers, M. A., Burri, D. J., Pfeil, M. P., ... & Yang, P. L. (2020). Hepatitis C virus NS3-4A protease regulates the lipid environment for RNA replication by cleaving host enzyme 24-dehydrocholesterol reductase. Journal of Biological Chemistry, 295(35), 12426-12436.

Simonen, P., Li, S., Chua, N. K., Lampi, A. M., Piironen, V., Lommi, J., ... & Gylling, H. (2020). Amiodarone disrupts cholesterol biosynthesis pathway and causes accumulation of circulating desmosterol by inhibiting 24‐dehydrocholesterol reductase. Journal of Internal Medicine, 288(5), 560-569.

Müller, C., Hemmers, S., Bartl, N., Plodek, A., Körner, A., Mirakaj, V., ... & Bracher, F. (2017). New chemotype of selective and potent inhibitors of human delta 24-dehydrocholesterol reductase. European Journal of Medicinal Chemistry, 140, 305-320.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-DHCR24 Recombinant Antibody (CBXS-1259)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry