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Mouse Anti-DUSP4 Recombinant Antibody (1D12) (CBMAB-D1900-YC)

Provided herein is a Mouse monoclonal antibody, which recognizes human DUSP4 protein. The antibody can be used for immunoassay techniques, such as ELISA, WB, IF.
See all DUSP4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D12
Antibody Isotype
IgG3, κ
Application
ELISA, WB, IF

Basic Information

Specificity
Human
Antibody Isotype
IgG3, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Dual Specificity Phosphatase 4
Introduction
DUSP4 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported.
Entrez Gene ID
1846
UniProt ID
Q13115
Alternative Names
Dual Specificity Phosphatase 4; MAP Kinase Phosphatase 2; Mitogen-Activated Protein Kinase Phosphatase 2; Dual Specificity Protein Phosphatase HVH2; VH1 Homologous Phosphatase 2; MKP-2; MKP2; Serine/Threonine Specific Protein Phosphatase;
Research Area
Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.
Biological Process
Dephosphorylation Source: UniProtKB
Endoderm formation Source: GO_Central
Inactivation of MAPK activity Source: UniProtKB
Negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
Peptidyl-threonine dephosphorylation Source: UniProtKB
Peptidyl-tyrosine dephosphorylation Source: UniProtKB
Protein dephosphorylation Source: GO_Central
Cellular Location
Nucleus
PTM
Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.

Su, H., Jiang, M., Senevirathne, C., Aluri, S., Zhang, T., Guo, H., ... & Zhao, X. (2021). Methylation of dual-specificity phosphatase 4 controls cell differentiation. Cell Reports, 36(4), 109421.

Gupta, A., Towers, C., Willenbrock, F., Brant, R., Hodgson, D. R., Sharpe, A., ... & Macaulay, V. M. (2020). Dual-specificity protein phosphatase DUSP4 regulates response to MEK inhibition in BRAF wild-type melanoma. British journal of cancer, 122(4), 506-516.

Ratsada, P., Hijiya, N., Hidano, S., Tsukamoto, Y., Nakada, C., Uchida, T., ... & Moriyama, M. (2020). DUSP4 is involved in the enhanced proliferation and survival of DUSP4-overexpressing cancer cells. Biochemical and Biophysical Research Communications, 528(3), 586-593.

Haddock, A. N., Labuzan, S. A., Haynes, A. E., Hayes, C. S., Kakareka, K. M., & Waddell, D. S. (2019). Dual-specificity phosphatase 4 is upregulated during skeletal muscle atrophy and modulates extracellular signal-regulated kinase activity. American Journal of Physiology-Cell Physiology, 316(4), C567-C581.

Prihantono, P., Usman, A. N., Binekada, C., Hatta, M., & Islam, A. A. (2019). Patterns of dual-specific phosphatase 4 mRNA expression before and after neoadjuvant chemotherapy in breast cancer. Asian Pacific Journal of Cancer Prevention: APJCP, 20(4), 1051.

Hofmann, S. R., Mäbert, K., Kapplusch, F., Russ, S., Northey, S., Beresford, M. W., ... & Hedrich, C. M. (2019). cAMP response element modulator α induces dual specificity protein phosphatase 4 to promote effector T cells in juvenile-onset lupus. The Journal of Immunology, 203(11), 2807-2816.

Ichimanda, M., Hijiya, N., Tsukamoto, Y., Uchida, T., Nakada, C., Akagi, T., ... & Moriyama, M. (2018). Downregulation of dual‐specificity phosphatase 4 enhances cell proliferation and invasiveness in colorectal carcinomas. Cancer Science, 109(1), 250-258.

Kobayashi, Y., Ito, K., Kanda, A., Tomoda, K., Mercado, N., & Barnes, P. J. (2017). Impaired dual-specificity protein phosphatase DUSP4 reduces corticosteroid sensitivity. Molecular Pharmacology, 91(5), 475-481.

Dougherty, J. A., Kilbane Myers, J., Khan, M., Angelos, M. G., & Chen, C. A. (2017). Dual-specificity phosphatase 4 overexpression in cells prevents hypoxia/reoxygenation-induced apoptosis via the upregulation of eNOS. Frontiers in Cardiovascular Medicine, 4, 22.

Kang, X., Li, M., Zhu, H., Lu, X., Miao, J., Du, S., ... & Guan, W. (2017). DUSP4 promotes doxorubicin resistance in gastric cancer through epithelial-mesenchymal transition. Oncotarget, 8(55), 94028.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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