Rabbit Anti-DUSP4 Recombinant Antibody (D1904) (CBMAB-D1904-YC)

Name: Rabbit Anti-DUSP4 Recombinant Antibody (D1904)
SKU: CBMAB-D1904-YC
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-DUSP4 Recombinant Antibody (D1904) (CBMAB-D1904-YC-1) in WB

Western blot from the same gel (cropped for rearrangement) showing protein levels of IRS2 and DUSP4 in WT and Ehmt2K182R/K182R mouse livers normalized to GAPDH using ImageJ (WT- n=5, WT+ n=6, K182R- n=6, K182R+ n=5 biologically independent mice). ...View More

Lee, R. A., Chang, M., Yiv, N., Tsay, A., Tian, S., Li, D., ... & Wang, J. C. (2023). Transcriptional coactivation by EHMT2 restricts glucocorticoid-induced insulin resistance in a study with male mice. Nature communications, 14(1), 3143.

Rabbit Anti-DUSP4 Recombinant Antibody (D1904) (CBMAB-D1904-YC-2) in WB

DUSP4 protein levels following expression of MEKDD. Phosphorylation of ERK, JNK and p38 following MEK inhibition. ...View More

Tuano, N. K., Beesley, J., Manning, M., Shi, W., Perlaza-Jimenez, L., Malaver-Ortega, L. F., ... & Rosenbluh, J. (2023). CRISPR screens identify gene targets at breast cancer risk loci. Genome biology, 24(1), 59.

Rabbit Anti-DUSP4 Recombinant Antibody (D1904) (CBMAB-D1904-YC-3) in WB

Mutant melanoma cells (COLO829 and SKMEL28) were transfected with siRNA against DUSP4 or nontargeting control. 48 h later, cell lysates were analyzed by Western blot. ...View More

Gutierrez-Prat, N., Zuberer, H. L., Mangano, L., Karimaddini, Z., Wolf, L., Tyanova, S., ... & Vivanco, I. (2022). DUSP4 protects BRAF-and NRAS-mutant melanoma from oncogene overdose through modulation of MITF. Life Science Alliance, 5(9).

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D1904

Application

Immunogen

Synthetic peptide corresponding to residues surrounding Pro168 of human DUSP4 protein.

Host Species

Rabbit

Specificity

Human, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & HEPES

Preservative

Sodium Azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Dual Specificity Phosphatase 4

Introduction

DUSP4 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported.

Entrez Gene ID

1846

UniProt ID

Q13115

Alternative Names

Dual Specificity Phosphatase 4; MAP Kinase Phosphatase 2; Mitogen-Activated Protein Kinase Phosphatase 2; Dual Specificity Protein Phosphatase HVH2; VH1 Homologous Phosphatase 2; MKP-2; MKP2; Serine/Threonine Specific Protein Phosphatase;

Research Area

Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.

Biological Process

Dephosphorylation Source: UniProtKB
Endoderm formation Source: GO_Central
Inactivation of MAPK activity Source: UniProtKB
Negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
Peptidyl-threonine dephosphorylation Source: UniProtKB
Peptidyl-tyrosine dephosphorylation Source: UniProtKB
Protein dephosphorylation Source: GO_Central

Cellular Location

Nucleus

PTM

Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.

Su, H., Jiang, M., Senevirathne, C., Aluri, S., Zhang, T., Guo, H., ... & Zhao, X. (2021). Methylation of dual-specificity phosphatase 4 controls cell differentiation. Cell Reports, 36(4), 109421.

Gupta, A., Towers, C., Willenbrock, F., Brant, R., Hodgson, D. R., Sharpe, A., ... & Macaulay, V. M. (2020). Dual-specificity protein phosphatase DUSP4 regulates response to MEK inhibition in BRAF wild-type melanoma. British journal of cancer, 122(4), 506-516.

Ratsada, P., Hijiya, N., Hidano, S., Tsukamoto, Y., Nakada, C., Uchida, T., ... & Moriyama, M. (2020). DUSP4 is involved in the enhanced proliferation and survival of DUSP4-overexpressing cancer cells. Biochemical and Biophysical Research Communications, 528(3), 586-593.

Haddock, A. N., Labuzan, S. A., Haynes, A. E., Hayes, C. S., Kakareka, K. M., & Waddell, D. S. (2019). Dual-specificity phosphatase 4 is upregulated during skeletal muscle atrophy and modulates extracellular signal-regulated kinase activity. American Journal of Physiology-Cell Physiology, 316(4), C567-C581.

Prihantono, P., Usman, A. N., Binekada, C., Hatta, M., & Islam, A. A. (2019). Patterns of dual-specific phosphatase 4 mRNA expression before and after neoadjuvant chemotherapy in breast cancer. Asian Pacific Journal of Cancer Prevention: APJCP, 20(4), 1051.

Hofmann, S. R., Mäbert, K., Kapplusch, F., Russ, S., Northey, S., Beresford, M. W., ... & Hedrich, C. M. (2019). cAMP response element modulator α induces dual specificity protein phosphatase 4 to promote effector T cells in juvenile-onset lupus. The Journal of Immunology, 203(11), 2807-2816.

Ichimanda, M., Hijiya, N., Tsukamoto, Y., Uchida, T., Nakada, C., Akagi, T., ... & Moriyama, M. (2018). Downregulation of dual‐specificity phosphatase 4 enhances cell proliferation and invasiveness in colorectal carcinomas. Cancer Science, 109(1), 250-258.

Kobayashi, Y., Ito, K., Kanda, A., Tomoda, K., Mercado, N., & Barnes, P. J. (2017). Impaired dual-specificity protein phosphatase DUSP4 reduces corticosteroid sensitivity. Molecular Pharmacology, 91(5), 475-481.

Dougherty, J. A., Kilbane Myers, J., Khan, M., Angelos, M. G., & Chen, C. A. (2017). Dual-specificity phosphatase 4 overexpression in cells prevents hypoxia/reoxygenation-induced apoptosis via the upregulation of eNOS. Frontiers in Cardiovascular Medicine, 4, 22.

Kang, X., Li, M., Zhu, H., Lu, X., Miao, J., Du, S., ... & Guan, W. (2017). DUSP4 promotes doxorubicin resistance in gastric cancer through epithelial-mesenchymal transition. Oncotarget, 8(55), 94028.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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For research use only. Not intended for any clinical use.

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