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Mouse Anti-ELAVL1 (AA 1-101) Recombinant Antibody (CBFYE-0197) (CBMAB-E0441-FY)

This product is mouse antibody that recognizes ELAVL1. The antibody CBFYE-0197 can be used for immunoassay techniques such as: IF, WB.
See all ELAVL1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-0197
Antibody Isotype
IgG1, κ
Application
IF, WB

Basic Information

Immunogen
ELAVL1 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-101

Target

Full Name
ELAV Like RNA Binding Protein 1
Introduction
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.
Entrez Gene ID
1994
UniProt ID
Q15717
Alternative Names
ELAV Like RNA Binding Protein 1; ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R); Embryonic Lethal, Abnormal Vision, Drosophila, Homolog-Like 1; Hu Antigen R; Hu-Antigen R; HUR
Research Area
RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969).

Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity).

Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:8626503, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:14731398).

Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503).

With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity).

Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010).
Biological Process
3'-UTR-mediated mRNA stabilization Source: UniProtKB
mRNA stabilization Source: UniProtKB
Negative regulation of gene silencing by miRNA Source: UniProtKB
Positive regulation of translation Source: MGI
Posttranscriptional gene silencing Source: Ensembl
Protein homooligomerization Source: UniProtKB
Protein import into nucleus Source: Ensembl
Regulation of stem cell population maintenance Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm; Stress granule. Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288). Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462).Localizes to the stress granules in the presence of PLEKHN1 (By similarity).
PTM
Phosphorylated by MAPKAPK2 (PubMed:14517288). Phosphorylated by PRKCD (PubMed:18285462).
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.

Chakraborty, A., Cadix, M., Relier, S., Taricco, N., Alaeitabar, T., Devaux, A., ... & Dutertre, M. (2022). Compartment-specific and ELAVL1-coordinated regulation of intronic polyadenylation isoforms by doxorubicin. Genome Research, 32(7), 1271-1284.

Guo, Q., Ren, H., Li, M., Liu, L., Li, W., Zhang, J., ... & Zheng, W. (2022). CMLD-2 Attenuates Myofibroblast Activation and Bleomycin-Induced Pulmonary Fibrosis in Mice through Antagonizing ELAVL1-Mediated Osteopontin mRNA Stabilization. bioRxiv.

Zhang, Q., Huang, X. M., Liao, J. X., Dong, Y. K., Zhu, J. L., He, C. C., ... & Tian, J. Y. (2021). LncRNA HOTAIR promotes neuronal damage through facilitating NLRP3 mediated-pyroptosis activation in Parkinson’s disease via regulation of miR-326/ELAVL1 axis. Cellular and Molecular Neurobiology, 41(8), 1773-1786.

Chen, H. Y., Xiao, Z. Z., Ling, X., Xu, R. N., Zhu, P., & Zheng, S. Y. (2021). ELAVL1 is transcriptionally activated by FOXC1 and promotes ferroptosis in myocardial ischemia/reperfusion injury by regulating autophagy. Molecular Medicine, 27(1), 1-14.

Rothamel, K., Arcos, S., Kim, B., Reasoner, C., Lisy, S., Mukherjee, N., & Ascano, M. (2021). ELAVL1 primarily couples mRNA stability with the 3′ UTRs of interferon-stimulated genes. Cell reports, 35(8), 109178.

Filippova, N., & Nabors, L. B. (2020). ELAVL1 role in cell fusion and tunneling membrane nanotube formations with implication to treat glioma heterogeneity. Cancers, 12(10), 3069.

Ling, Z., Chen, L., & Zhao, J. (2020). m6A-dependent up-regulation of DRG1 by METTL3 and ELAVL1 promotes growth, migration, and colony formation in osteosarcoma. Bioscience Reports, 40(4).

Zhang, Z., Yao, Z., Wang, L., Ding, H., Shao, J., Chen, A., ... & Zheng, S. (2018). Activation of ferritinophagy is required for the RNA-binding protein ELAVL1/HuR to regulate ferroptosis in hepatic stellate cells. Autophagy, 14(12), 2083-2103.

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For research use only. Not intended for any clinical use.

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