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  • Mouse Anti-ERLIN1 Recombinant Antibody (CBFYE-1197)

Mouse Anti-ERLIN1 Recombinant Antibody (CBFYE-1197) (V2LY-0825-LY719)

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Basic Information

Host Animal
Mouse
Clone
CBFYE-1197
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 250-277 within an internal region of human SPFH1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
ER Lipid Raft Associated 1
Entrez Gene ID
Human10613
Mouse226144
Rat293939
UniProt ID
HumanO75477
MouseQ91X78
RatB1WBY7
Research Area
Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. Binds cholesterol and may promote ER retention of the SCAP-SREBF complex (PubMed:24217618).

(Microbial infection) Required early in hepatitis C virus (HCV) infection to initiate RNA replication, and later in the infection to support infectious virus production.
Biological Process
Cholesterol metabolic process Source: UniProtKB-KW
Negative regulation of cholesterol biosynthetic process Source: UniProtKB
Negative regulation of fatty acid biosynthetic process Source: UniProtKB
SREBP signaling pathway Source: UniProtKB
Ubiquitin-dependent ERAD pathway Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane.
Involvement in disease
Spastic paraplegia 62, autosomal recessive (SPG62):
A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
Topology
Cytoplasmic: 1-7
Helical: 8-28
Lumenal: 29-348

Zhu, Z. Y., Li, Z. Y., Zhang, C., Liu, X. L., Tian, W. T., & Cao, L. (2022). A novel homozygous mutation in ERLIN1 gene causing spastic paraplegia 62 and literature review. European Journal of Medical Genetics, 104608.

Gao, X., Bonzerato, C. G., & Wojcikiewicz, R. J. (2022). Binding of the erlin1/2 complex to the third intralumenal loop of IP3R1 triggers its ubiquitin-proteasomal degradation. Journal of Biological Chemistry, 102026.

Manganelli, V., Matarrese, P., Antonioli, M., Gambardella, L., Vescovo, T., Gretzmeier, C., ... & Garofalo, T. (2021). Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs. Autophagy, 17(9), 2528-2548.

Huang, S. S., Toufiq, M., Saraiva, L. R., Van Panhuys, N., Chaussabel, D., & Garand, M. (2021). Transcriptome and literature mining highlight the differential expression of ERLIN1 in immune cells during sepsis. Biology, 10(8), 755.

Sleptcov, A., Nazarenko, M., Kazantsev, A., Lebedev, I., Barbarash, O., & Puzyrev, V. (2019). Assessment Of Sfmbt1, Prkra, And Erlin1 Copy Number Variations In Patients With Carotid Atherosclerosis Using Droplet Digital Pcr. Atherosclerosis, 287, e165-e166.

Whitten-Bauer, C., Chung, J., Gómez-Moreno, A., Gomollón-Zueco, P., Huber, M. D., Gerace, L., & Garaigorta, U. (2019). The host factor erlin-1 is required for efficient hepatitis C virus infection. Cells, 8(12), 1555.

Tunca, C., Akçimen, F., Coşkun, C., Gündoğdu-Eken, A., Kocoglu, C., Çevik, B., ... & Başak, A. N. (2018). ERLIN1 mutations cause teenage-onset slowly progressive ALS in a large Turkish pedigree. European Journal of Human Genetics, 26(5), 745-748.

Wright, F. A., Bonzerato, C. G., Sliter, D. A., & Wojcikiewicz, R. J. (2018). The erlin2 T65I mutation inhibits erlin1/2 complex–mediated inositol 1, 4, 5-trisphosphate receptor ubiquitination and phosphatidylinositol 3-phosphate binding. Journal of Biological Chemistry, 293(40), 15706-15714.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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