Mouse Anti-FADS3 Recombinant Antibody (CBXF-2823) (V2LY-0825-LY1288)

Name: Mouse Anti-FADS3 Recombinant Antibody (CBXF-2823)
SKU: V2LY-0825-LY1288
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-2823

Application

ELISA, WB

Immunogen

Partial recombinant corresponding to aa16-113 from human FADS3.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fatty Acid Desaturase 3

Entrez Gene ID

3995

UniProt ID

Q9Y5Q0

Research Area

Mammals have different sphingoid bases that differ in their length and/or pattern of desaturation and hydroxyl groups. The predominant sphingoid base in mammalian ceramides is sphing-4-enine (sphingosine or SPH) which has a trans desaturation at carbon 4. FADS3 is a ceramide desaturase that introduces a cis double bond between carbon 14 and carbon 15 of the SPH-containing ceramides, producing sphinga-4,14-dienine-containing ceramides (SPD ceramides). SPD ceramides occur widely in mammalian tissues and cells. Due to their unusual structure containing a cis double bond, SPD ceramides may have an opposite, negative role in lipid microdomain formation relative to conventional ceramides (PubMed:31916624).

FADS3 also acts as a methyl-end fatty acyl coenzyme A (CoA) desaturase that introduces a cis double bond between the preexisting double bond and the terminal methyl group of the fatty acyl chain. Desaturates (11E)-octadecenoate (trans-vaccenoate, the predominant trans fatty acid in human milk) at carbon 13 to generate (11E,13Z)-octadecadienoate (also known as conjugated linoleic acid 11E,13Z-CLA).

Biological Process

Sphingolipid metabolic process Source: UniProtKB-UniPathway
Unsaturated fatty acid biosynthetic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1-133
Helical: 134-154
Lumenal: 155-159
Helical: 160-180
Cytoplasmic: 181-263
Helical: 264-284
Lumenal: 285-306
Helical: 307-327
Cytoplasmic: 328-445

Karsai, G., Lone, M., Kutalik, Z., Brenna, J. T., Li, H., Pan, D., ... & Hornemann, T. (2020). FADS3 is a Δ14Z sphingoid base desaturase that contributes to gender differences in the human plasma sphingolipidome. Journal of Biological Chemistry, 295(7), 1889-1897.

Jojima, K., Edagawa, M., Sawai, M., Ohno, Y., & Kihara, A. (2020). Biosynthesis of the anti‐lipid‐microdomain sphingoid base 4, 14‐sphingadiene by the ceramide desaturase FADS3. The FASEB Journal, 34(2), 3318-3335.

Rioux, V., & Legrand, P. (2019). Fatty Acid Desaturase 3 (FADS3) Is a Specific∆ 13-Desaturase of Ruminant trans-Vaccenic Acid. Lifestyle Genomics, 12(1-6), 18-24.

Yang, H. T., Wang, R. Y., Huang, S. Y., Huang, C. L., & Su, K. P. (2018). Genetic polymorphisms of FADS1, FADS2, and FADS3 and fatty acid profiles in subjects received methadone maintenance therapy. Prostaglandins, Leukotrienes and Essential Fatty Acids, 136, 117-121.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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