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Mouse Anti-FAM122A Recombinant Antibody (3E9) (CBMAB-F1961-CQ)

This product is a mouse antibody that recognizes FAM122A. The antibody 3E9 can be used for immunoassay techniques such as: ELISA, WB.
See all FAM122A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3E9
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
FAM122A (NP_612206.3, 1 a.a.-287 a.a) full-length recombinant protein with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
family with sequence similarity 122A
Introduction
FAM122A (Family With Sequence Similarity 122A) is a Protein Coding gene. An important paralog of this gene is FAM122B.
Entrez Gene ID
116224
UniProt ID
Q96E09
Alternative Names
Family With Sequence Similarity 122A; C9orf42; Chromosome 9 Open Reading Frame 42; Protein FAM122A;
Research Area
Acts as an inhibitor of serine/threonine-protein phosphatase 2A (PP2A) activity (PubMed:27588481, PubMed:33108758).

Potentiates ubiquitin-mediated proteasomal degradation of serine/threonine-protein phosphatase 2A catalytic subunit alpha (PPP2CA) (PubMed:27588481).

Inhibits PP2A-mediated dephosphorylation of WEE1, promoting ubiquitin-mediated proteolysis of WEE1, thereby releasing G2/M checkpoint (PubMed:33108758).
Biological Process
Mitotic G2/M transition checkpoint Source: UniProtKB
Positive regulation of cell growth Source: UniProtKB
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. The CHEK1-mediated Ser-37 phosphorylated form is sequestered by 14-3-3 proteins in the cytoplasm and fails to translocate to the nucleus, where it otherwise inhibits serine/threonine-protein phosphatase 2A.
PTM
CHEK1-mediated phosphorylation at Ser-37 negatively regulates its ability to inhibit serine/threonine-protein phosphatase 2A (PP2A) activity. Phosphorylation leads to its release from the PP2A complex and its sequestration by 14-3-3 proteins in the cytoplasm resulting in its inability to translocate to the nucleus, where it otherwise inhibits PP2A.

Liu, M. H., Chen, J., Yang, Y. S., Wang, Y. Q., Chen, G. Q., Zhang, Y., & Huang, Y. (2021). FAM122A promotes acute myeloid leukemia cell growth through inhibiting PP2A activity and sustaining MYC expression. haematologica, 106(3), 903.

Liu, M. H., Zhang, X. C., Chen, J., Yang, Y. S., Wang, Y. Q., Zheng, J. K., ... & Huang, Y. (2021). FAM122A is required for hematopoietic stem cell function. Leukemia, 35(7), 2130-2134.

Li, F., Kozono, D., Deraska, P., Branigan, T., Dunn, C., Zheng, X. F., ... & D’Andrea, A. D. (2020). CHK1 inhibitor blocks phosphorylation of FAM122A and promotes replication stress. Molecular cell, 80(3), 410-422.

Zhou, Y., Shi, W. Y., He, W., Yan, Z. W., Liu, M. H., Chen, J., ... & Huang, Y. (2020). FAM122A supports the growth of hepatocellular carcinoma cells and its deletion enhances doxorubicin-induced cytotoxicity. Experimental Cell Research, 387(1), 111714.

Wang, Y. Q., Yang, Y. S., Chen, J., Liu, M. H., Chen, G. Q., & Huang, Y. (2020). FAM122A maintains DNA stability possibly through the regulation of topoisomerase IIα expression. Experimental Cell Research, 396(1), 112242.

Chen, J., Zhou, Q., Liu, M. H., Yang, Y. S., Wang, Y. Q., Huang, Y., & Chen, G. Q. (2020). FAM122A Inhibits Erythroid Differentiation through GATA1. Stem cell reports, 15(3), 721-734.

Fan, F., Zhao, J., Liu, Y., Zhao, H., Weng, L., Li, Q., ... & Xu, Y. (2018). Identifying the SUMO1 modification of FAM122A leading to the degradation of PP2A-Cα by ubiquitin-proteasome system. Biochemical and biophysical research communications, 500(3), 676-681.

Deraska, P., Reavis, H., Labe, S., D'Andrea, A., & Kozono, D. (2018). Whole-genome CRISPR/Cas9 screen of the CHK1 inhibitor prexasertib implicates FAM122A loss as a potential resistance mechanism. Cancer Research, 78(13_Supplement), 4287-4287.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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