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Mouse Anti-FBXO18 Recombinant Antibody (2353C1a) (CBMAB-F0823-CQ)

This product is a mouse antibody that recognizes FBXO18. The antibody 2353C1a can be used for immunoassay techniques such as: WB, Dot.
See all FBXO18 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2353C1a
Antibody Isotype
IgG1
Application
WB, Dot

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
F-box protein 18
Introduction
This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbx class. It contains an F-box motif and seven conserved helicase motifs, and has both DNA-dependent ATPase and DNA unwinding activities. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
Entrez Gene ID
84893
UniProt ID
Q8NFZ0
Alternative Names
F-Box Protein, Helicase, 18; F-Box Only Protein 18; F-Box DNA Helicase 1; Fbx18; HFBH1; FBH1; F-Box Only Protein, Helicase, 18; EC 3.6.4.12;
Research Area
3'-5' DNA helicase and substrate-recognition component of the SCF(FBH1) E3 ubiquitin ligase complex that plays a key role in response to stalled/damaged replication forks (PubMed:11956208, PubMed:23393192).

Involved in genome maintenance by acting as an anti-recombinogenic helicase and preventing extensive strand exchange during homologous recombination: promotes RAD51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination (PubMed:17724085, PubMed:19736316).

Also promotes cell death and DNA double-strand breakage in response to replication stress: together with MUS81, promotes the endonucleolytic DNA cleavage following prolonged replication stress via its helicase activity, possibly to eliminate cells with excessive replication stress (PubMed:23319600, PubMed:23361013).

Plays a major role in remodeling of stalled DNA forks by catalyzing fork regression, in which the fork reverses and the two nascent DNA strands anneal (PubMed:25772361).

In addition to the helicase activity, also acts as the substrate-recognition component of the SCF(FBH1) E3 ubiquitin ligase complex, a complex that mediates ubiquitination of RAD51, leading to regulate RAD51 subcellular location (PubMed:25585578).
Biological Process
Cell death Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
DNA catabolic process, endonucleolytic Source: MGI
Double-strand break repair via homologous recombination Source: UniProtKB
Negative regulation of chromatin binding Source: Ensembl
Negative regulation of double-strand break repair via homologous recombination Source: UniProtKB
Positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
Positive regulation of protein phosphorylation Source: MGI
Protein ubiquitination Source: UniProtKB
Recombinational repair Source: GO_Central
Replication fork processing Source: UniProtKB
Replication fork protection Source: UniProtKB
Response to intra-S DNA damage checkpoint signaling Source: MGI
Cellular Location
Nucleus; Chromosome. Accumulates at sites of DNA damage or replication stress (PubMed:19736316, PubMed:23677613). PCNA is required for localization to DNA damage sites (PubMed:23677613). Localizes to the nucleoplasm in absence of DNA damage (PubMed:23677613).
Involvement in disease
Defects in FBH1 are frequently observed in melanomas, resulting in increased survival in response to replicative stress. Its inactivation may play a role in oncogenic transformation.
PTM
Ubiquitinated (PubMed:23393192, PubMed:23677613). Ubiquitination by the DCX(DTL) complex, also named CRL4(CDT2), leading to its degradation: ubiquitination takes place after its localization to DNA damage sites, possibly to facilitate the translesion synthesis (TLS) pathway (PubMed:23677613).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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