Mouse Anti-FBXW8 Recombinant Antibody (CBXF-1732) (CBMAB-F3267-CQ)

Name: Mouse Anti-FBXW8 Recombinant Antibody (CBXF-1732)
SKU: CBMAB-F3267-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-1732

Application

WB, IP, IF, ELISA

Specificity

Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

F-Box And WD Repeat Domain Containing 8

Introduction

This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

Entrez Gene ID

Mouse	231672
Rat	304522

UniProt ID

Mouse	Q8BIA4
Rat	P0DL28

Alternative Names

F-Box And WD Repeat Domain Containing 8; F-Box Only Protein 29; F-Box And WD-40 Domain-Containing Protein 8; F-Box And WD-40 Domain Protein 8; FBXO29; FBXW6;

Research Area

Substrate-recognition component of a Cul7-RING ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain (PubMed:21572988).

Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed:18498745).

The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation (PubMed:24362026).

Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions (PubMed:24793695).

Biological Process

Cell population proliferation Source: UniProtKB
Golgi organization Source: UniProtKB
Labyrinthine layer blood vessel development Source: Ensembl
Positive regulation of dendrite morphogenesis Source: UniProtKB
Positive regulation of transcription factor catabolic process Source: Ensembl
Protein ubiquitination Source: UniProtKB
Spongiotrophoblast layer development Source: Ensembl

Cellular Location

Golgi apparatus; Perinuclear region. Colocalizes with CUL7 at the Golgi apparatus in neurons.

Zhang, Y., Cui, L., Chen, W., Belviso, B. D., Yu, B., & Shen, Y. (2022). Structure-based drug design of potential inhibitors of FBXW8, the substrate recognition component of Cullin-RING ligase 7. Molecular Diversity, 1-15.

Zheng, Z., Hong, D., Zhang, X., Chang, Y., Sun, N., Lin, Z., ... & Jin, H. (2021). uc. 77-Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation. Frontiers in oncology, 11, 673223.

Islam, S., Dutta, P., Chopra, K., Sahay, O., Rapole, S., Chauhan, R., & Santra, M. K. (2021). Co-operative binding of SKP1, Cullin1 and Cullin7 to FBXW8 results in Cullin1-SKP1-FBXW8-Cullin7 functional complex formation that monitors cellular function of β-TrCP1. International journal of biological macromolecules, 190, 233-243.

Islam, S., Dutta, P., Chopra, K., Rapole, S., Chauhan, R., & Santra, M. K. (2021). FBXW8 regulates G1 and S phases of cell cycle progression by restricting β‐TrCP1 function. The FEBS Journal, 288(18), 5474-5497.

Gao, S., & Zhao, D. (2020). Fbxw8 protein promotes the proliferation and metastasis of ESCC. In E3S Web of Conferences (Vol. 185, p. 04010). EDP Sciences.

Shi, D., Zhang, Y., Lu, R., & Zhang, Y. (2018). The long non-coding RNA MALAT1 interacted with miR-218 modulates choriocarcinoma growth by targeting Fbxw8. Biomedicine & Pharmacotherapy, 97, 543-550.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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