Mouse Anti-FGA Recombinant Antibody (CBXF-2019) (CBMAB-F0738-CQ)

Name: Mouse Anti-FGA Recombinant Antibody (CBXF-2019)
SKU: CBMAB-F0738-CQ
Rating: 5 (1 reviews)

See all FGA antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-2019

Application

ELISA, WB, IHC-P

Immunogen

Native protein

Specificity

Human

Antibody Isotype

IgA

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

TBS, pH7.4, 1% BSA, 40% Glycerol

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Fibrinogen Alpha Chain

Introduction

This gene encodes the alpha subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, the encoded preproprotein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing.

Entrez Gene ID

2243

UniProt ID

P02671

Alternative Names

Fibrinogen Alpha Chain; Fibrinogen, A Alpha Polypeptide; Fib2;

Research Area

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Blood coagulation, common pathway Source: BHF-UCL
Blood coagulation, fibrin clot formation Source: UniProtKB
Cell-matrix adhesion Source: BHF-UCL
Cellular protein-containing complex assembly Source: BHF-UCL
Fibrinolysis Source: UniProtKB
Induction of bacterial agglutination Source: CACAO
Innate immune response Source: UniProtKB-KW
Negative regulation of blood coagulation, common pathway Source: BHF-UCL
Negative regulation of endothelial cell apoptotic process Source: BHF-UCL
Negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
Plasminogen activation Source: UniProtKB
Platelet aggregation Source: BHF-UCL
Positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
Positive regulation of exocytosis Source: BHF-UCL
Positive regulation of heterotypic cell-cell adhesion Source: BHF-UCL
Positive regulation of peptide hormone secretion Source: BHF-UCL
Positive regulation of protein secretion Source: BHF-UCL
Positive regulation of substrate adhesion-dependent cell spreading Source: BHF-UCL
Positive regulation of vasoconstriction Source: BHF-UCL
Protein-containing complex assembly Source: UniProtKB
Protein polymerization Source: BHF-UCL
Response to calcium ion Source: BHF-UCL

Cellular Location

Secreted

Involvement in disease

Congenital afibrinogenemia (CAFBN):
The disease is caused by variants affecting the gene represented in this entry. The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias. Rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen.
Amyloidosis 8 (AMYL8):
A form of hereditary generalized amyloidosis. Clinical features include extensive visceral amyloid deposits, renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. There is no involvement of the nervous system.
Dysfibrinogenemia, congenital (DYSFIBRIN):
A disorder characterized by qualitative abnormalities (dysfibrinogenemia) of the circulating fibrinogen. Affected individuals are frequently asymptomatic, but some patients have bleeding diathesis, thromboembolic complications, or both. In some cases, dysfibrinogenemia is associated with low circulating fibrinogen levels (hypodysfibrinogenemia).

PTM

The alpha chain is normally not N-glycosylated (PubMed:23151259), even though glycosylation at Asn-686 was observed when a fragment of the protein was expressed in insect cells (PubMed:9689040). It is well known that heterologous expression of isolated domains can lead to adventitious protein modifications. Besides, glycosylation at Asn-686 is supported by large-scale glycoproteomics studies (PubMed:16335952 and PubMed:19159218), but the evidence is still quite tenuous. Most likely, Asn-686 is not glycosylated in the healthy human body, or only with low efficiency.
O-glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylated by FAM20C in the extracellular medium.

Li, X., Liu, Y., Cheng, J., Xia, Y., Fan, K., Liu, Y., & Liu, P. F. (2022). Identification and expression analysis of a fibrinogen alpha chain-like gene in Atlantic salmon (Salmo salar). Aquaculture Reports, 22, 100919.

Rezaei-Tavirani, M., Nejad, M. R., Arjmand, B., Tavirani, S. R., Razzaghi, M., & Mansouri, V. (2021). Fibrinogen Dysregulation is a Prominent Process in Fatal Conditions of COVID-19 Infection; a Proteomic Analysis. Archives of Academic Emergency Medicine, 9(1).

Liu, L., Song, J., Li, J., Huang, N., Yang, J., Hu, S., ... & Wang, W. (2020). Isoform 1 of Fibrinogen Alpha Chain Precursor is a Potential Biomarker for Steroid‐Induced Osteonecrosis of the Femoral Head. PROTEOMICS–Clinical Applications, 14(6), 1900099.

Wang, M., Zhang, G., Zhang, Y., Cui, X., Wang, S., Gao, S., ... & Liu, R. (2020). Fibrinogen alpha chain knockout promotes tumor growth and metastasis through integrin–AKT signaling pathway in lung cancer. Molecular Cancer Research, 18(7), 943-954.

Li, H., Ma, R. Q., Cheng, H. Y., Ye, X., Zhu, H. L., & Chang, X. H. (2020). Fibrinogen alpha chain promotes the migration and invasion of human endometrial stromal cells in endometriosis through focal adhesion kinase/protein kinase B/matrix metallopeptidase 2 pathway. Biology of Reproduction, 103(4), 779-790.

Chen, Y., Li, H., Cheng, H. Y., Rui-Qiong, M., Ye, X., Cui, H., ... & Chang, X. H. (2019). Fibrinogen alpha chain is up-regulated and affects the pathogenesis of endometriosis. Reproductive BioMedicine Online, 39(6), 893-904.

Chapman, J., & Dogan, A. (2019). Fibrinogen alpha amyloidosis: insights from proteomics. Expert review of proteomics, 16(9), 783-793.

Wypasek, E., Klukowska, A., Zdziarska, J., Zawilska, K., Treliński, J., Iwaniec, T., ... & Undas, A. (2019). Genetic and clinical characterization of congenital fibrinogen disorders in Polish patients: Identification of three novel fibrinogen gamma chain mutations. Thrombosis Research, 182, 133-140.

Salomon, O., Barel, O., Eyal, E., Ganor, R. S., Kleinbaum, Y., & Shohat, M. (2019). c. 259A> C in the fibrinogen gene of alpha chain (FGA) is a fibrinogen with thrombotic phenotype. The Application of Clinical Genetics, 12, 27.

Cai, R., Li, Y., Wang, W., Gao, X., Liu, M., Diao, Y., ... & Feng, Q. (2018). A novel fibrinogen variant in a Chinese pedigree with congenital dysfibrinogenemia caused by FGA P. Arg38Thr mutation: a case report. Medicine, 97(40).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-FGA Recombinant Antibody (CBXF-2019)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

FGA Matched Antibody Pair (424) (CAT#: APMAB-424LY)

FGA Matched Antibody Pair (425) (CAT#: APMAB-425LY)

Related Products

Mouse Anti-FGA Recombinant Antibody (CBFYH-0191) (CAT#: CBMAB-H0515-FY)

Rabbit Anti-FGA Recombinant Antibody (CBXF-2233) (CAT#: CBMAB-F0712-CQ)

Mouse Anti-FGA Recombinant Antibody (20B2) (CAT#: CBMAB-F2852-CQ)

Mouse Anti-FGA Recombinant Antibody (CBXF-3696) (CAT#: CBMAB-F0691-CQ)

Mouse Anti-FGA Recombinant Antibody (CBXF-0042) (CAT#: CBMAB-F1438-CQ)

Mouse Anti-D-dimer Recombinant Antibody (DD3) (CAT#: CBMAB-D2065-YC)

Mouse Anti-FGA Recombinant Antibody (1D6) (CAT#: CBMAB-AO461LY)

Mouse Anti-D-dimer Recombinant Antibody (3C6) (CAT#: CBMAB-D2057-YC)

Mouse Anti-FGA Recombinant Antibody (CBXF-2964) (CAT#: CBMAB-F3670-CQ)

Rat Anti-FGA Recombinant Antibody (CBXF-0516) (CAT#: CBMAB-F1831-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry