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Rabbit Anti-FLOT1 Monoclonal Antibody (EP446Y) (CBMAB-1265-YC)

Provided herein is a rabbit monoclonal antibody against Human FLOT1. The antibody, clone EP446Y, can be used for immunoassay techniques, such as WB.
See all FLOT1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
EP446Y
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
A synthetic peptide corresponding to residues in human Flotillin-1
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Flotillin 1
Introduction
FLOT1 localizes to the caveolae, which are small domains on the inner cell membranes. FLOT1 plays a role in vesicle trafficking and cell morphology.
Entrez Gene ID
Human10211
Mouse14251
UniProt ID
HumanO75955
MouseO08917
Alternative Names
flotillin-1; integral membrane component of caveolae
Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Biological Process
Axonogenesis Source: Ensembl
Cellular response to exogenous dsRNA Source: UniProtKB
dsRNA transport Source: UniProtKB
Extracellular matrix disassembly Source: UniProtKB
Membrane raft assembly Source: UniProtKB
Plasma membrane raft assembly Source: UniProtKB
Positive regulation of cell adhesion molecule production Source: UniProtKB
Positive regulation of cell-cell adhesion mediated by cadherin Source: UniProtKB
Positive regulation of cell junction assembly Source: UniProtKB
Positive regulation of cytokine production Source: UniProtKB
Positive regulation of endocytosis Source: UniProtKB
Positive regulation of heterotypic cell-cell adhesion Source: UniProtKB
Positive regulation of interferon-beta production Source: UniProtKB
Positive regulation of myoblast fusion Source: UniProtKB
Positive regulation of NF-kappaB transcription factor activity Source: ARUK-UCL
Positive regulation of protein binding Source: UniProtKB
Positive regulation of protein phosphorylation Source: UniProtKB
Positive regulation of skeletal muscle tissue development Source: UniProtKB
Positive regulation of synaptic transmission, dopaminergic Source: UniProtKB
Positive regulation of toll-like receptor 3 signaling pathway Source: UniProtKB
Protein kinase C signaling Source: UniProtKB
Protein localization to membrane raft Source: UniProtKB
Protein localization to plasma membrane Source: UniProtKB
Protein stabilization Source: UniProtKB
Regulation of neurotransmitter uptake Source: SynGO
Regulation of receptor internalization Source: UniProtKB
Regulation of Rho protein signal transduction Source: Ensembl
Response to endoplasmic reticulum stress Source: UniProtKB
Cellular Location
Endosome; Cell membrane; Caveola; Melanosome; Membrane raft. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity).

Mao, S., Qian, Y., Wei, W., Lin, X., Ling, Q., Ye, W., ... & Jin, J. (2023). FLOT1 knockdown inhibits growth of AML cells through triggering apoptosis and pyroptosis. Annals of Hematology, 1-13.

Cai, S., Zhou, Y., Pan, Y., Liu, P., Yu, K., & Chen, S. (2021). Long non-coding RNA A1BG-AS1 promotes tumorigenesis in breast cancer by sponging microRNA-485-5p and consequently increasing expression of FLOT1 expression. Human cell, 34(5), 1517-1531.

Cao, Y., He, Q., Qi, Z., Zhang, Y., Lu, L., Xue, J., ... & Li, R. (2020). Dynamics and endocytosis of Flot1 in Arabidopsis require CPI1 function. International Journal of Molecular Sciences, 21(5), 1552.

Kan, X. Q., Li, Y. B., He, B., Cheng, S., Wei, Y., & Sun, J. (2020). MiR-1294 acts as a tumor inhibitor in cervical cancer by regulating FLOT1 expression. Journal of Biological Regulators and Homeostatic Agents, 34(2).

Lv, D., Xiang, Y., Yang, Q., Yao, J., & Dong, Q. (2020). Long non-coding RNA TUG1 promotes cell proliferation and inhibits cell apoptosis, Autophagy in clear cell renal cell carcinoma via MiR-31-5p/FLOT1 axis. OncoTargets and therapy, 13, 5857.

Liu, C., Shang, Z., Ma, Y., Ma, J., & Song, J. (2019). HOTAIR/miR-214-3p/FLOT1 axis plays an essential role in the proliferation, migration, and invasion of hepatocellular carcinoma. International Journal of Clinical and Experimental Pathology, 12(1), 50.

Zhong, J., Li, S., Zeng, W., Li, X., Gu, C., Liu, J., & Luo, X. J. (2019). Integration of GWAS and brain eQTL identifies FLOT1 as a risk gene for major depressive disorder. Neuropsychopharmacology, 44(9), 1542-1551.

Zhang, L., Mao, Y., Mao, Q., Fan, W., Xu, L., Chen, Y., ... & Wang, J. (2019). FLOT1 promotes tumor development, induces epithelial–mesenchymal transition, and modulates the cell cycle by regulating the Erk/Akt signaling pathway in lung adenocarcinoma. Thoracic cancer, 10(4), 909-917.

Chen, K., Wu, Q., Hu, K., Yang, C., Wu, X., Cheung, P., & Williams, K. J. (2018). Suppression of hepatic FLOT1 (flotillin-1) by type 2 diabetes mellitus impairs the disposal of remnant lipoproteins via syndecan-1. Arteriosclerosis, thrombosis, and vascular biology, 38(1), 102-113.

Sun, X., Luo, L. H., Zhong, K. Z., & Li, J. R. (2018). LncRNA PCAT29 suppresses cell proliferation, invasion, and migration in renal carcinoma by regulating FLOT1. Clinical Surgery Research Communications, 2(3), 34-41.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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