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Mouse Anti-GLTSCR2 Recombinant Antibody (5A8) (CBMAB-A3474-LY)

The product is antibody recognizes GLTSCR2. The antibody 5A8 immunoassay techniques such as: WB, ELISA.
See all GLTSCR2 antibodies
Published Data
Fig. 1 Western blot
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Summary

Host Animal
Mouse
Specificity
Human
Clone
5A8
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
GLTSCR2 (NP_056525, 402 a.a. ~ 478 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
glioma tumor suppressor candidate region gene 2
Entrez Gene ID
29997
UniProt ID
P43220
Alternative Names
PICT-1; PICT1
Function
Nucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis (PubMed:24120868).

In ribosome biogenesis, may also play a role in rRNA transcription (PubMed:27729611).

Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions (PubMed:21741933, PubMed:24120868, PubMed:27829214).

DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation (PubMed:24120868, PubMed:27829214).

It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation (PubMed:22522597).

Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway (PubMed:15355975, PubMed:16971513, PubMed:27729611).

May also inhibit cell proliferation and increase apoptosis through its interaction with NF2 (PubMed:21167305).

May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation (PubMed:25818168).

Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex (PubMed:25956029).

May also regulate cellular aerobic respiration (PubMed:24556985).

In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of DDX58/RIG-1 (PubMed:27824081).
Biological Process
Cellular response to DNA damage stimulus Source: UniProtKB
Cellular response to hypoxia Source: UniProtKB
DNA repair Source: UniProtKB
Mitotic G2 DNA damage checkpoint signaling Source: UniProtKB
Negative regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
Negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Negative regulation of protein-containing complex assembly Source: UniProtKB
Negative regulation of protein kinase B signaling Source: UniProtKB
Negative regulation of signal transduction by p53 class mediator Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Negative regulation of transcription of nucleolar large rRNA by RNA polymerase I Source: UniProtKB
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Positive regulation of protein K63-linked deubiquitination Source: UniProtKB
Protein localization to nucleolus Source: Ensembl
Protein localization to nucleoplasm Source: UniProtKB
Protein stabilization Source: UniProtKB
Regulation of aerobic respiration Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Regulation of cell cycle Source: UniProtKB
Regulation of protein phosphorylation Source: UniProtKB
Regulation of RIG-I signaling pathway Source: UniProtKB
Regulation of signal transduction by p53 class mediator Source: UniProtKB
Ribosomal large subunit assembly Source: HGNC
rRNA processing Source: GO_Central
Cellular Location
Nucleolus; Nucleoplasm. In the nucleolus may be more specifically localized to the fibrillar center (PubMed:27729611). Mainly nucleolar it relocalizes to the nucleoplasm under specific conditions including ribosomal stress enabling it to interact and regulate nucleoplasmic proteins like p53/TP53 (PubMed:22522597, PubMed:24923447, PubMed:27323397, PubMed:26903295). Also detected in the cytosol (PubMed:24923447, PubMed:27824081).
PTM
Ubiquitin-mediated proteasomal degradation is regulated by c-JUN. It is associated with relocalization to the nucleoplasm and decreased homooligomerization.
Phosphorylated upon DNA damage probably by ATM and DNA-PK; may regulate NOP53 degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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