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Mouse Anti-GNAI1 Recombinant Antibody (CBLG1-1404) (CBMAB-G4096-LY)

This product is antibody recognizes GNAI1. The antibody CBLG1-1404 immunoassay techniques such as: WB.
See all GNAI1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLG1-1404
Antibody Isotype
IgG1, κ
Application
WB

Basic Information

Immunogen
GNAI1 (AAH26326, 1 a.a. ~ 354 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Introduction
Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Entrez Gene ID
2770
UniProt ID
P63096
Alternative Names
G Protein Subunit Alpha I1; Guanine Nucleotide Binding Protein (G Protein); Alpha Inhibiting Activity Polypeptide 1; Adenylate Cyclase-Inhibiting G Alpha Protein; Gi1 Protein Alpha Subunit; Guanine Nucleotide-Binding Protein G(I) Subunit Alpha-1; Gi;
Function
Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541).

Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity).

The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed:17635935).

Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed:22327364).
Biological Process
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: Reactome
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB
Cellular response to forskolin Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Negative regulation of synaptic transmission Source: Ensembl
Positive regulation of protein localization to cell cortex Source: UniProtKB
Regulation of cAMP-mediated signaling Source: UniProtKB
Regulation of mitotic spindle organization Source: UniProtKB
Response to peptide hormone Source: BHF-UCL
Cellular Location
Centrosome; Nucleus; Cell membrane; Cytoplasm; Cell cortex; Membrane. Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody (PubMed:17635935). Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane.
PTM
Myristoylation at Gly-2 is required for membrane anchoring before palmitoylation.By similarity
Palmitoylation at Cys-3 varies with membrane lipid composition.By similarity
(Microbial infection) Deamidated at Gln-204 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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