Mouse Anti-GPR143 Recombinant Antibody (EG1378) (CBMAB-EN1657-LY)

Name: Mouse Anti-GPR143 Recombinant Antibody (EG1378)
SKU: CBMAB-EN1657-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG1378

Application

ELISA, IP, IF, WB

Immunogen

Amino acids 97-126 near the N-terminus of mouse OA1.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G protein-coupled receptor 143

Introduction

This gene encodes a protein that binds to heterotrimeric G proteins and is targeted to melanosomes in pigment cells. This protein is thought to be involved in intracellular signal transduction mechanisms. Mutations in this gene cause ocular albinism type 1, also referred to as Nettleship-Falls type ocular albinism, a severe visual disorder. A related pseudogene has been identified on chromosome Y.

Entrez Gene ID

Human	4935
Mouse	18241

UniProt ID

Human	P51810
Mouse	P70259

Alternative Names

OA1; NYS6

Biological Process

Calcium-mediated signaling using intracellular calcium source Source: UniProtKB
Eye pigment biosynthetic process Source: ProtInc
G protein-coupled receptor signaling pathway Source: UniProtKB
Melanosome localization Source: UniProtKB
Melanosome organization Source: UniProtKB
Melanosome transport Source: UniProtKB
Phosphatidylinositol-mediated signaling Source: UniProtKB
Regulation of calcium-mediated signaling Source: UniProtKB
Signal transduction Source: ProtInc
Visual perception Source: ProtInc

Cellular Location

Apical cell membrane; Lysosome membrane; Melanosome membrane. Distributed throughout the endo-melanosomal system but most of endogenous protein is localized in unpigmented stage II melanosomes. Its expression on the apical cell membrane is sensitive to tyrosine (PubMed:18828673).

Involvement in disease

Albinism ocular 1 (OA1):
Form of albinism affecting only the eye. Pigment of the hair and skin is normal or only slightly diluted. Eyes may be severely affected with photophobia and reduced visual acuity. Nystagmus or strabismus are often associated. The irides and fundus are depigmented.
Nystagmus congenital X-linked 6 (NYS6):
A condition defined as conjugated, spontaneous and involuntary ocular oscillations that appear at birth or during the first three months of life. Other associated features may include mildly decreased visual acuity, strabismus, astigmatism, and occasionally head nodding.

Topology

Extracellular: 1-28
Helical: 29-49
Cytoplasmic: 50-78
Helical: 79-99
Extracellular: 100-124
Helical: 125-145
Cytoplasmic: 146-149
Helical: 150-170
Extracellular: 171-191
Helical: 192-212
Cytoplasmic: 213-248
Helical: 249-269
Extracellular: 270-292
Helical: 293-313
Cytoplasmic: 314-404

PTM

Glycosylated.
Phosphorylated.

Bueschbell, B., Manga, P., & Schiedel, A. C. (2022). The many faces of G protein-coupled receptor 143, an atypical intracellular receptor. Frontiers in Molecular Biosciences, 9.

Nakano, M., Koga, M., Hashimoto, T., Matsushita, N., Masukawa, D., Mizuno, Y., ... & Goshima, Y. (2022). Right ventricular overloading is attenuated in monocrotaline-induced pulmonary hypertension model rats with a disrupted Gpr143 gene, the gene that encodes the 3, 4-l-dihydroxyphenyalanine (l-DOPA) receptor. Journal of Pharmacological Sciences, 148(2), 214-220.

Bueschbell, B., Manga, P., Penner, E., & Schiedel, A. C. (2021). Evidence for protein–protein interaction between dopamine receptors and the G protein-coupled receptor 143. International Journal of Molecular Sciences, 22(15), 8328.

Mao, X., Chen, M., Yu, Y., Liu, Q., Yuan, S., & Fan, W. (2021). Identification of a novel GPR143 mutation in a large Chinese family with isolated foveal hypoplasia. BMC ophthalmology, 21, 1-6.

Gao, X., Liu, T., Cheng, X., Dai, A., Liu, W., Li, R., & Zhang, M. (2020). A novel GPR143 mutation in a Chinese family with X‑linked ocular albinism type 1. Molecular Medicine Reports, 21(1), 240-248.

Jiang, J., Yang, L., Li, H., Huang, L., & Li, N. (2019). Evaluation of the iris thickness changes for the Chinese families with GPR143 gene mutations. Experimental Eye Research, 189, 107819.

Li, N. (2018). Analysis of GPR143 Gene Mutations in Five Chinese Families with the Ocular Albinism Type I. Investigative Ophthalmology & Visual Science, 59(9), 1030-1030.

Jung, J. H., Oh, E. H., Shin, J. H., Kim, H. S., Choi, S. Y., Choi, K. D., ... & Choi, J. H. (2018). Identification of a novel GPR143 mutation in X-linked ocular albinism with marked intrafamilial phenotypic variability. Journal of genetics, 97, 1479-1484.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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