Mouse Anti-HLA-F Recombinant Antibody (CBT2862) (V2LY-0625-LY1771)

Name: Mouse Anti-HLA-F Recombinant Antibody (CBT2862)
SKU: V2LY-0625-LY1771
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-HLA-F Recombinant Antibody (CBT2862) (V2LY-0625-LY1771) in ELISA

Mouse Anti-HLA-F Recombinant Antibody (CBT2862) (V2LY-0625-LY1771) in WB

Mouse Anti-HLA-F Recombinant Antibody (CBT2862) (V2LY-0625-LY1771) in FC

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBT2862

Application

WB, FC

Immunogen

Purified recombinant fragment of human HLA-F (AA: 22-305) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Major Histocompatibility Complex, Class I, F

Entrez Gene ID

3134

UniProt ID

P30511

Function

Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors (PubMed:28636952).

Upon interaction with activating KIR3DS1 receptor on NK cells, triggers NK cell degranulation and anti-viral cytokine production (PubMed:27455421).

Through interaction with KIR3DL2 receptor, inhibits NK and T cell effector functions (PubMed:24018270).

May interact with other MHC class I OCs to cross-present exogenous viral, tumor or minor histompatibility antigens to cytotoxic CD8+ T cells, triggering effector and memory responses (PubMed:23851683).

May play a role in inflammatory responses in the peripheral nervous system. Through interaction with KIR3DL2, may protect motor neurons from astrocyte-induced toxicity (PubMed:26928464).

Biological Process

Antigen processing and presentation of endogenous peptide antigen via MHC class Ib Source: UniProtKB
Antigen processing and presentation of exogenous peptide antigen via MHC class Ib Source: UniProtKB
Antigen processing and presentation of peptide antigen via MHC class I Source: UniProtKB-KW
Negative regulation of natural killer cell cytokine production Source: UniProtKB
Negative regulation of natural killer cell degranulation Source: UniProtKB
Negative regulation of natural killer cell mediated cytotoxicity Source: UniProtKB
Negative regulation of neuron death Source: UniProtKB
Negative regulation of T cell cytokine production Source: UniProtKB
Positive regulation of natural killer cell cytokine production Source: UniProtKB
Positive regulation of natural killer cell degranulation Source: UniProtKB
Positive regulation of T cell mediated cytotoxicity Source: UniProtKB

Cellular Location

Lysosome membrane; Early endosome membrane; Cell membrane. For cross-presentation transits from the cell surface through endosomal pathway to lysosomes, where the peptide is generated from internalized exogenous antigen.

Topology

Extracellular: 22-305
Helical: 306-329
Cytoplasmic: 330-346

PTM

N-glycosylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-HLA-F Recombinant Antibody (CBT2806) (CAT#: V2LY-0625-LY1772)

Mouse Anti-HLA-F Monoclonal Antibody (CBFYH-3143) (CAT#: CBMAB-H2408-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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