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Mouse Anti-INCENP Recombinant Antibody (58-217) (CBMAB-I1404-YY)

This product is Mouse antibody that recognizes INCENP. The antibody 58-217 can be used for immunoassay techniques such as: IHC-P, IF, ELISA, ICC, IP, WB
See all INCENP antibodies
Published Data
Fig.1 Untreated or siRNA transfected (siBub1, siGl2 for control) HeLa S3 cells were synchronized by thymidine block and released for 10 hr, as indicated.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
58-217
Antibody Isotype
IgG1
Application
IHC-P, IF, ELISA, ICC, IP, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Inner Centromere Protein
Introduction
In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994
Entrez Gene ID
3621
UniProt ID
Q9UK53
Alternative Names
Inner Centromere Protein
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules (PubMed:15316025, PubMed:12925766, PubMed:27332895).
The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity).
Activates AURKB and AURKC (PubMed:27332895).
Required for localization of CBX5 to mitotic centromeres (PubMed:21346195).
Controls the kinetochore localization of BUB1 (PubMed:16760428).
Biological Process
Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Histone phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Meiotic spindle midzone assemblyManual Assertion Based On ExperimentIBA:GO_Central
Metaphase plate congressionManual Assertion Based On ExperimentIBA:GO_Central
Mitotic cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic sister chromatid segregationIEA:InterPro
Positive regulation of protein serine/threonine kinase activityManual Assertion Based On ExperimentIBA:GO_Central
Regulation of mitotic cytokinesisIEA:InterPro
Cellular Location
Nucleus; Cytoplasm, cytoskeleton, spindle; Midbody; Chromosome, centromere, kinetochore. Colocalized at synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocenters. Colocalizes with AURKB at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone (By similarity).
Colocalizes with AURKB at mitotic chromosomes (PubMed:11453556).
Localizes to inner kinetochore (PubMed:16760428).
Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis (PubMed:15316025).
Cocalizes to the equatorial cell cortex at anaphase (PubMed:11453556).
PTM
Phosphorylation by AURKB or AURKC at its C-terminal part is important for AURKB or AURKC activation by INCENP.

Redouane, S., Charoute, H., Harmak, H., Malki, A., Barakat, A., & Rouba, H. (2022). Computational study of the potential impact of AURKC missense SNPs on AURKC-INCENP interaction and their correlation to macrozoospermia. Journal of Biomolecular Structure and Dynamics, 1-20.

Shukla, S., Bhattacharya, A., Agarwal, P., Malik, N., Duraisamy, K., Sri Rangan, N., ... & Kumar, A. (2022). Intrinsic disorder in CENP-ACse4 tail and its chaperone facilitates synergistic association for kinetochore stabilization. bioRxiv, 2022-08.

Petsalaki, E., & Zachos, G. (2021). An ATM-CHK2-INCENP pathway prevents chromatin breakage by regulating the abscission checkpoint. Molecular & Cellular Oncology, 8(2), 1877999.

Zhang, C., Xie, L., Fu, Y., Yang, J., & Cui, Y. (2020). lncRNA MIAT promotes esophageal squamous cell carcinoma progression by regulating miR‐1301‐3p/INCENP axis and interacting with SOX2. Journal of cellular physiology, 235(11), 7933-7944.

Petsalaki, E., & Zachos, G. (2020). An ATM–Chk2–INCENP pathway activates the abscission checkpoint. Journal of Cell Biology, 220(2), e202008029.

García-Rodríguez, L. J., Kasciukovic, T., Denninger, V., & Tanaka, T. U. (2019). Aurora B-INCENP localization at centromeres/inner kinetochores is required for chromosome bi-orientation in budding yeast. Current Biology, 29(9), 1536-1544.

Abdul Azeez, K. R., Chatterjee, S., Yu, C., Golub, T. R., Sobott, F., & Elkins, J. M. (2019). Structural mechanism of synergistic activation of Aurora kinase B/C by phosphorylated INCENP. Nature communications, 10(1), 3166.

Sun, M., Veschi, V., Bagchi, S., Xu, M., Mendoza, A., Liu, Z., & Thiele, C. J. (2019). Targeting the chromosomal passenger complex subunit INCENP induces polyploidization, apoptosis, and senescence in neuroblastoma. Cancer research, 79(19), 4937-4950.

Papini, D., Fant, X., Ogawa, H., Desban, N., Samejima, K., Feizbakhsh, O., ... & Ruchaud, S. (2019). Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1. Journal of cell science, 132(21), jcs234401.

Feng, H., Raasholm, M., Moosmann, A., Campsteijn, C., & Thompson, E. M. (2019). Switching of INCENP paralogs controls transitions in mitotic chromosomal passenger complex functions. Cell Cycle, 18(17), 2006-2025.

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For research use only. Not intended for any clinical use.

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