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Rabbit Anti-ITPK1 Recombinant Antibody (EG1703) (CBMAB-EN2039-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
EG1703
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:10000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human ITPK1.
Specificity
Human, Mouse
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
inositol-tetrakisphosphate 1-kinase
Introduction
This gene encodes an enzyme that belongs to the inositol 1,3,4-trisphosphate 5/6-kinase family. This enzyme regulates the synthesis of inositol tetraphosphate, and downstream products, inositol pentakisphosphate and inositol hexakisphosphate. Inositol metabolism plays a role in the development of the neural tube. Disruptions in this gene are thought to be associated with neural tube defects. A pseudogene of this gene has been identified on chromosome X.
Entrez Gene ID
Human3705
Mouse217837
UniProt ID
HumanQ13572
MouseQ8BYN3
Alternative Names
ITRPK1
Function
Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3 (PubMed:11042108, PubMed:8662638).
Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5 (PubMed:11042108).
This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway (PubMed:11042108, PubMed:8662638).
Also acts as an inositol polyphosphate phosphatase that dephosphorylates Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain (PubMed:11909533, PubMed:12925536, PubMed:17616525).
Plays an important role in MLKL-mediated necroptosis. Produces highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which bind to MLKL mediating the release of an N-terminal auto-inhibitory region leading to its activation. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis (PubMed:17616525).
Biological Process
Blood coagulationTAS:Reactome
Inositol phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Inositol trisphosphate metabolic processIEA:InterPro
Necroptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Neural tube developmentIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Apical plasma membrane; Cytosol
PTM
Acetylation by EP300 and CREBBP destabilizes ITPK1, and down-regulates enzymatic activity. Deacetylated by SIRT1.
More Infomation

Pullagurla, N. J., Shome, S., Yadav, R., & Laha, D. (2023). ITPK1 Regulates Jasmonate-Controlled Root Development in Arabidopsis thaliana. Biomolecules, 13(9), 1368.

Riemer, E., Qiu, D., Laha, D., Harmel, R. K., Gaugler, P., Gaugler, V., ... & Giehl, R. F. (2021). ITPK1 is an InsP6/ADP phosphotransferase that controls phosphate signaling in Arabidopsis. Molecular Plant, 14(11), 1864-1880.

Gulabani, H., Goswami, K., Walia, Y., Roy, A., Noor, J. J., Ingole, K. D., ... & Bhattacharjee, S. (2021). Arabidopsis inositol polyphosphate kinases IPK1 and ITPK1 modulate crosstalk between SA-dependent immunity and phosphate-starvation responses. Plant Cell Reports, 1-17.

Whitfield, H., White, G., Sprigg, C., Riley, A. M., Potter, B. V., Hemmings, A. M., & Brearley, C. A. (2020). An ATP-responsive metabolic cassette comprised of inositol tris/tetrakisphosphate kinase 1 (ITPK1) and inositol pentakisphosphate 2-kinase (IPK1) buffers diphosphosphoinositol phosphate levels. Biochemical Journal, 477(14), 2621-2638.

Laha, N. P., Dhir, Y. W., Giehl, R. F., Schäfer, E. M., Gaugler, P., Shishavan, Z. H., ... & Schaaf, G. (2020). ITPK1-dependent inositol polyphosphates regulate auxin responses in Arabidopsis thaliana. Biorxiv, 2020-04.

Laha, D., Parvin, N., Hofer, A., Giehl, R. F., Fernandez-Rebollo, N., von Wirén, N., ... & Schaaf, G. (2019). Arabidopsis ITPK1 and ITPK2 have an evolutionarily conserved phytic acid kinase activity. ACS chemical biology, 14(10), 2127-2133.

Desfougères, Y., Wilson, M. S., Laha, D., Miller, G. J., & Saiardi, A. (2019). ITPK1 mediates the lipid-independent synthesis of inositol phosphates controlled by metabolism. Proceedings of the National Academy of Sciences, 116(49), 24551-24561.

Kuo, H. F., Hsu, Y. Y., Lin, W. C., Chen, K. Y., Munnik, T., Brearley, C. A., & Chiou, T. J. (2018). Arabidopsis inositol phosphate kinases IPK 1 and ITPK 1 constitute a metabolic pathway in maintaining phosphate homeostasis. The Plant Journal, 95(4), 613-630.

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For research use only. Not intended for any clinical use.

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