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Mouse Anti-KLF4 Recombinant Antibody (4E5C3) (CBMAB-K1108-LY)

This product is antibody recognizes KLF4. The antibody 4E5C3 immunoassay techniques such as: ELISA, EIA, WB.
See all KLF4 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
4E5C3
Antibody Isotype
IgG1
Application
ELISA, EIA, WB

Basic Information

Immunogen
A 20 amino acid synthetic peptide near the carboxy terminus of Human KLF4 (AM50014CP- N)
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Kruppel Like Factor 4
Introduction
This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Entrez Gene ID
Human9314
Mouse16600
Rat114505
UniProt ID
HumanO43474
MouseQ60793
RatQ923V7
Alternative Names
Kruppel Like Factor 4; Epithelial Zinc Finger Protein EZF; Gut-Enriched Krueppel-Like Factor; Kruppel-Like Factor 4 (Gut); Gut Kruppel-Like Factor; GKLF; EZF; Endothelial Kruppel-Like Zinc Finger Protein; Krueppel-Like Factor 4;
Function
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Biological Process
Canonical Wnt signaling pathwayIEA:Ensembl
Cellular response to growth factor stimulusManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular response to laminar fluid shear stressManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to leukemia inhibitory factorIEA:Ensembl
Defense response to tumor cellManual Assertion Based On ExperimentIMP:ARUK-UCL
Epidermal cell differentiationIEA:Ensembl
Epidermis morphogenesisIEA:Ensembl
Fat cell differentiationISS:BHF-UCL
Mesodermal cell fate determinationManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of angiogenesisManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of cell migration involved in sprouting angiogenesisManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of cell population proliferationManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of chemokine (C-X-C motif) ligand 2 productionManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of gene expressionManual Assertion Based On ExperimentIGI:BHF-UCL
Negative regulation of heterotypic cell-cell adhesionManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of inflammatory responseManual Assertion Based On ExperimentTAS:BHF-UCL
Negative regulation of interleukin-8 productionManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of leukocyte adhesion to arterial endothelial cellManual Assertion Based On ExperimentIGI:BHF-UCL
Negative regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of response to cytokine stimulusManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cellular protein metabolic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of core promoter bindingIEA:Ensembl
Positive regulation of gene expressionManual Assertion Based On ExperimentIGI:BHF-UCL
Positive regulation of hemoglobin biosynthetic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of nitric oxide biosynthetic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of pri-miRNA transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of protein metabolic processManual Assertion Based On ExperimentIGI:BHF-UCL
Positive regulation of sprouting angiogenesisManual Assertion Based On ExperimentIGI:BHF-UCL
Positive regulation of telomerase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:BHF-UCL
Post-embryonic camera-type eye developmentIEA:Ensembl
Post-embryonic hemopoiesisManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of axon regenerationIEA:Ensembl
Regulation of blastocyst developmentIEA:Ensembl
Regulation of cell differentiationISS:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Response to retinoic acidIEA:Ensembl
Stem cell population maintenanceISS:UniProtKB
Transcription, DNA-templatedIEA:Ensembl
Cellular Location
Nucleus
PTM
Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis.
Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6; deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis.

He, Z., He, J., & Xie, K. (2023). KLF4 transcription factor in tumorigenesis. Cell Death Discovery, 9(1), 118.

Li, J. C., Chen, Q. H., Jian, R., Zhou, J. R., Xu, Y., Lu, F., ... & Zhang, H. (2021). The partial role of KLF4 and KLF5 in gastrointestinal tumors. Gastroenterology Research and Practice, 2021.

Subbalakshmi, A. R., Sahoo, S., McMullen, I., Saxena, A. N., Venugopal, S. K., Somarelli, J. A., & Jolly, M. K. (2021). KLF4 induces mesenchymal–epithelial transition (MET) by suppressing multiple EMT-inducing transcription factors. Cancers, 13(20), 5135.

Yu, S., Guo, J., Sun, Z., Lin, C., Tao, H., Zhang, Q., ... & Chen, Z. (2021). BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation. Cell death & disease, 12(2), 197.

Zhang, J., Wu, Y., Lin, L., & Liu, X. (2020). Transcription Factor KLF4: A Potential Biomarker of Non-Small Cell Lung Cancertranscription Factor KLF4: A Potential Biomarker of Non-Small Cell Lung Cancer.

Huang, X., Wang, C., Zhou, X., Wang, J., Xia, K., Yang, B., ... & Chen, Q. (2020). Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury. CNS Neuroscience & Therapeutics, 26(9), 940-951.

Dhaliwal, N. K., Abatti, L. E., & Mitchell, J. A. (2019). KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control. Genes & Development, 33(15-16), 1069-1082.

Di Giammartino, D. C., Kloetgen, A., Polyzos, A., Liu, Y., Kim, D., Murphy, D., ... & Apostolou, E. (2019). KLF4 is involved in the organization and regulation of pluripotency-associated three-dimensional enhancer networks. Nature cell biology, 21(10), 1179-1190.

Morales-Martinez, M., Valencia-Hipolito, A., Vega, G. G., Neri, N., Nambo, M. J., Alvarado, I., ... & Vega, M. I. (2019). Regulation of Krüppel-Like Factor 4 (KLF4) expression through the transcription factor Yin-Yang 1 (YY1) in non-Hodgkin B-cell lymphoma. Oncotarget, 10(22), 2173.

Cheng, Z., Zou, X., Jin, Y., Gao, S., Lv, J., Li, B., & Cui, R. (2018). The role of KLF4 in Alzheimer’s disease. Frontiers in cellular neuroscience, 12, 325.

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For research use only. Not intended for any clinical use.

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