Mouse Anti-KLHL22 Recombinant Antibody (CBT4753) (V2LY-0625-LY2524)

Name: Mouse Anti-KLHL22 Recombinant Antibody (CBT4753)
SKU: V2LY-0625-LY2524
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT4753

Antibody Isotype

IgG1

Application

WB, ICC

Basic Information

Immunogen

Purified recombinant fragment of human KLHL22 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Kelch Like Family Member 22

Entrez Gene ID

84861

UniProt ID

Q53GT1

Function

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (PubMed:19995937, PubMed:23455478).
The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy (PubMed:29769719).

Biological Process

Cell divisionManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to leucineManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic sister chromatid segregationManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic spindle assembly checkpoint signalingManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of autophagyManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of cell growthManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of TORC1 signalingManual Assertion Based On ExperimentIMP:UniProtKB
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Protein monoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm, cytosol; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Nucleus; Lysosome. Mainly cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase (PubMed:23455478).
Localizes to the nucleus upon amino acid starvation (PubMed:29769719).
Relocalizes to the cytosol and associates with lysosomes when amino acids are available (PubMed:29769719).

Involvement in disease

Defects in KLHL22 has been found in a patient with isolated coloboma, a defect of the eye characterized by the absence of ocular structures due to abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Isolated colobomas may be associated with an abnormally small eye (microphthalmia) or small cornea.

More Infomation

References

Gao, C., Sun, C., Zhang, L., Zhang, H., Yu, R., & Kang, C. (2023). Design and Screening of KLHL22 Inhibitors by Homology Modeling, Molecular Docking, and Molecular Dynamics Simulation. Letters in Drug Design & Discovery, 20(3), 291-303.

Teng, F., Wang, Y., Liu, M., Tian, S., Stjepanovic, G., & Su, M. Y. (2023). Cryo-EM structure of the KLHL22 E3 ligase bound to an oligomeric metabolic enzyme. Structure.

Kong, X., Shu, X., Wang, J., Liu, D., Ni, Y., Zhao, W., ... & Wang, Z. (2022). Fine-tuning of mTOR signaling by the UBE4B-KLHL22 E3 ubiquitin ligase cascade in brain development. Development, 149(24), dev201286.

Song, Y., Yuan, H., Wang, J., Wu, Y., Xiao, Y., & Mao, S. (2020). KLHL22 regulates the EMT and proliferation in colorectal cancer cells in part via the Wnt/β-catenin signaling pathway. Cancer Management and Research, 3981-3993.

Zhou, X. A., Zhou, J., Zhao, L., Yu, G., Zhan, J., Shi, C., ... & Wang, J. (2020). KLHL22 maintains PD-1 homeostasis and prevents excessive T cell suppression. Proceedings of the National Academy of Sciences, 117(45), 28239-28250.

Chen, J., Ou, Y., Yang, Y., Li, W., Xu, Y., Xie, Y., & Liu, Y. (2018). KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing. Nature, 557(7706), 585-589.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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