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Mouse Anti-M. tuberculosis GroEL Recombinant Antibody (CBMY-C0407) (CBMAB-V208-C0419-YY)

See all M. tuberculosis GroEL antibodies
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Basic Information

Host Animal
Mouse
Clone
CBMY-C0407
Application
ELISA
Immunogen
Recombinant protein corresponding to full length Chaperonin GroEL
Specificity
M. tuberculosis
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Mycobacterium tuberculosis GroEL
Introduction
Molecular chaperones are helper proteins, which play essential roles in folding, assembly, and transport of several cellular proteins. Chaperonins, a subclass of the molecular chaperones, are homo- or hetero-oligomeric proteins, which carry out the substrate protein folding in a sequestered cavity. One of the best-characterized chaperonins is the 60-kDa chaperonin of Escherichia coli, GroEL. Chaperonins are highly conserved proteins and are known to interact with nonnative substrate proteins in an ATP-dependent manner. The E. coli genome possesses a single copy of groEL, arranged in an operonic arrangement with groES and expressed under all growth conditions. GroEL forms a cylindrical assembly with two heptameric rings and functions in coordination with the heptameric GroES.
Function
Prevents aggregation of substrate proteins and promotes their refolding (PubMed:15327959, PubMed:19717599, PubMed:21094166).
In vitro, activity may be independent of the presence or absence of the GroES co-chaperonin or ATP (PubMed:15327959).
Shows weak ATPase activity (PubMed:15327959, PubMed:32812602).
Involved in copper homeostasis (PubMed:32808291, PubMed:32812602).
Binds copper and may help maintaining copper homeostasis when copper is present in excess, notably in the macrophage phagosome, by acting as a metal storage protein (PubMed:32808291, PubMed:32812602).
Could be involved in copper resistance during mycobacterial biofilm formation (PubMed:32812602).
Protects from copper stress in vitro (PubMed:32808291).
Can also bind other metals, but binds copper with relatively higher affinity compared to nickel and cobalt (PubMed:32808291).
May play an important role in survival under low aeration by affecting the expression of genes known for hypoxia response (PubMed:26822628).
Biological Process
Chaperone cofactor-dependent protein refoldingManual Assertion Based On ExperimentIBA:GO_Central
DNA protectionManual Assertion Based On ExperimentIDA:MTBBASE
Nucleoid organizationManual Assertion Based On ExperimentIDA:MTBBASE
Positive regulation of transcription regulatory region DNA bindingManual Assertion Based On ExperimentIDA:CAFA
Protein foldingManual Assertion Based On ExperimentIBA:GO_Central
Protein refoldingManual Assertion Based On ExperimentIDA:CAFA
Response to heatManual Assertion Based On ExperimentIEP:MTBBASE
Cellular Location
Cytoplasm
PTM
Phosphorylated on Thr-25 and Thr-54 by PknF (PubMed:19201798).
Phosphorylated on Ser-393 by an unknown kinase (PubMed:19717599).
N-terminus is acetylated by RimI.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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