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Mouse Anti-MCM5 (AA 367-582 ) Recombinant Antibody (CBFYM-0236) (CBMAB-M0288-FY)

This product is mouse antibody that recognizes MCM5. The antibody CBFYM-0236 can be used for immunoassay techniques such as: WB, IHC, IF.
See all MCM5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-0236
Antibody Isotype
IgG2b
Application
WB, IHC, IF

Basic Information

Immunogen
His-tagged human Mcm5 (amino acids 367-582)
Specificity
Human
Antibody Isotype
IgG2b
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 367-582

Target

Full Name
Minichromosome Maintenance Complex Component 5
Introduction
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Entrez Gene ID
4174
UniProt ID
P33992
Alternative Names
Minichromosome Maintenance Complex Component 5; CDC46 Homolog; P1-CDC46; CDC46; MCM5 Minichromosome Maintenance Deficient 5, Cell Division Cycle 46 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 5 (Cell Division Cycle 46)
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Biological Process
Cell cycle Source: UniProtKB-KW
DNA replication Source: ProtInc
DNA replication initiation Source: GO_Central
DNA unwinding involved in DNA replication Source: ComplexPortal
Double-strand break repair via break-induced replication Source: GO_Central
Regulation of DNA-dependent DNA replication initiation Source: ComplexPortal
Cellular Location
Nucleus
cytosol
Other locations
Chromosome
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.
Involvement in disease
Meier-Gorlin syndrome 8 (MGORS8):
A form of Meier-Gorlin syndrome, a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal. MGORS8 inheritance is autosomal recessive.

Mathew, L., Janardhanan, M., Suresh, R., Savithri, V., Aravind, T., & Aiswarya, A. (2023). Minichromosome maintenance protein 5–a promising prognostic marker of oral epithelial dysplasias and oral squamous cell carcinomas. Journal of Oral and Maxillofacial Pathology, 27(4), 655-662.

Sun, M., Wang, T., Zhu, Y., Zhang, Y., Zhu, L., & Li, X. (2022). The high expression of minichromosome maintenance complex component 5 is an adverse prognostic factor in lung adenocarcinoma. BioMed Research International, 2022.

Hao, M., Wang, H., Zhang, C., Li, C., & Wang, X. (2020). Minichromosome maintenance protein 5 is an important pathogenic factor of oral squamous cell carcinoma. Oncology Letters, 20(4), 1-1.

Aporowicz, M., Czopnik, P., Kubicka, E., Piotrowska, A., Dziegiel, P., Bolanowski, M., & Domoslawski, P. (2019). Minichromosome maintenance proteins MCM-3, MCM-5, MCM-7, and Ki-67 as proliferative markers in adrenocortical tumors. Anticancer Research, 39(3), 1151-1159.

Everson, M., Magee, C., Alzoubaidi, D., Brogden, S., Graham, D., Lovat, L. B., ... & Haidry, R. (2019). Minichromosomal Maintenance Component Complex 5 (MCM5) as a Marker of Barrett’s Esophagus-Related Neoplasia: A Feasibility Study. Digestive Diseases and Sciences, 64, 2815-2822.

Zhao, M., Wang, T., Zhen, C., Wang, J., Pickering, C. R., Chen, J., ... & Zhou, G. (2019). Gain-of-function mutant p53 predisposes head and neck keratinocytes and squamous cell carcinoma cells to replicative stress and genomic instability through minichromosome maintenance complex component 5. Cancer Research, 79(13_Supplement), 5289-5289.

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For research use only. Not intended for any clinical use.

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