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Mouse Anti-MELK Recombinant Antibody (4D8) (CBMAB-A5419-LY)

The product is antibody recognizes MELK. The antibody 4D8 immunoassay techniques such as: ELISA.
See all MELK antibodies
Published Data
Fig.1 Immunoblot analysis monitoring MELK expression in MT4C5 cells stably expressing either non-target (Non-T) or MELK-specific shRNA (MELK-KD-2) (upper panel).
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Fig.2 Depletion of MELK delays HIV-1 CA disassembly.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
4D8
Antibody Isotype
IgG1, κ
Application
ELISA

Basic Information

Immunogen
MELK (AAH14039, 550 a.a. ~ 650 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
maternal embryonic leucine zipper kinase
Entrez Gene ID
9833
UniProt ID
Q14680
Alternative Names
HPK38; KIAA0175
Function
Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
Biological Process
Apoptotic process Source: UniProtKB
Cell population proliferation Source: UniProtKB
G2/M transition of mitotic cell cycle Source: UniProtKB
Hemopoiesis Source: UniProtKB
Intracellular signal transduction Source: GO_Central
Intrinsic apoptotic signaling pathway in response to oxidative stress Source: Ensembl
Neural precursor cell proliferation Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: GO_Central
Cellular Location
Cell membrane
Involvement in disease
Defects in MELK are associated with some cancers, such as brain or breast cancers. Expression is dramatically increased in aggressive undifferentiated tumors, correlating with poor patient outcome in breast and brain cancers, suggesting a role in tumor-initiating cells and proliferation via its function in cell proliferation regulation.
PTM
Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8 (Probable).

Tang, B. F., Yan, R. C., Wang, S. W., Zeng, Z. C., & Shi-Suo, D. (2023). Maternal embryonic leucine zipper kinase in tumor cell and tumor microenvironment: Emerging player and promising therapeutic opportunities. Cancer Letters, 216126.

Li, H., Gai, L., Wu, Z., & Li, F. (2022). Maternal embryonic leucine zipper kinase serves as a potential prognostic marker and leads to sorafenib chemoresistance modified by miR-142-5p in hepatocellular carcinoma. Molecular Biology Reports, 49(4), 3015-3024.

Nishiyama, T., Takada, T., Takeuchi, H., & Iwami, S. (2022). Maternal embryonic leucine zipper kinase (MELK) optimally regulates the HIV-1 uncoating process. Journal of Theoretical Biology, 545, 111152.

Guo, Z., & Zhu, Z. (2022). Comprehensive analysis to identify noncoding RNAs mediated upregulation of maternal embryonic leucine zipper kinase (MELK) correlated with poor prognosis in hepatocellular carcinoma. Aging (Albany NY), 14(9), 3973.

Chen, P., Wang, J., Wang, X., Chen, X., Li, C., & Tan, T. (2020). Cloning, tissue distribution, expression pattern, and function of porcine maternal embryonic leucine zipper kinase. Annals of Translational Medicine, 8(5).

Chen, L., Wei, Q., Bi, S., & Xie, S. (2020). Maternal embryonic leucine zipper kinase promotes tumor growth and metastasis via stimulating FOXM1 signaling in esophageal squamous cell carcinoma. Frontiers in oncology, 10, 10.

Perry, N. A., Fialkowski, K. P., Kaoud, T. S., Kaya, A. I., Chen, A. L., Taliaferro, J. M., ... & Iverson, T. M. (2019). Arrestin-3 interaction with maternal embryonic leucine-zipper kinase. Cellular signalling, 63, 109366.

Chlenski, A., Park, C., Dobratic, M., Salwen, H. R., Budke, B., Park, J. H., ... & Cohn, S. L. (2019). Maternal embryonic leucine zipper kinase (MELK), a potential therapeutic target for neuroblastoma. Molecular cancer therapeutics, 18(3), 507-516.

Zang, X., Qian, C., Ruan, Y., Xie, J., Luo, T., Xu, B., & Jiang, J. (2019). Higher maternal embryonic leucine zipper kinase mRNA expression level is a poor prognostic factor in non-small-cell lung carcinoma patients. Biomarkers in Medicine, 13(16), 1349-1361.

Maes, A., Maes, K., Vlummens, P., De Raeve, H., Devin, J., Szablewski, V., ... & De Bruyne, E. (2019). Maternal embryonic leucine zipper kinase is a novel target for diffuse large B cell lymphoma and mantle cell lymphoma. Blood Cancer Journal, 9(12), 87.

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For research use only. Not intended for any clinical use.

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