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Mouse Anti-MRE11 (AA 182-582) Recombinant Antibody (CBFYM-0043) (CBMAB-M0049-FY)

This product is mouse antibody that recognizes MRE11. The antibody CBFYM-0043 can be used for immunoassay techniques such as: ELISA, IF, IHC-P, IP, WB, FuncS.
See all MRE11 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBFYM-0043
Antibody Isotype
IgG1
Application
ELISA, IF, IHC-P, IP, WB, FuncS

Basic Information

Immunogen
Amino acids 182-582 of Mre11 expressed in E. coli
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 182-582

Target

Full Name
MRE11 Homolog, Double Strand Break Repair Nuclease
Introduction
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Entrez Gene ID
Human4361
Mouse17535
UniProt ID
HumanP49959
MouseQ61216
Alternative Names
MRE11 Homolog, Double Strand Break Repair Nuclease; MRE11 Homolog A, Double Strand Break Repair Nuclease; Double-Strand Break Repair Protein MRE11A; Meiotic Recombination 11 Homolog 1; Meiotic Recombination 11 Homolog A; AT-Like Disease; MRE11 Homolog 1; MRE11A; HNGS1; MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae); MRE11 Homolog, Double Strand Break Repair Nuclease A
Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289).

The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11 (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289).

RAD50 may be required to bind DNA ends and hold them in close proximity (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289).

This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289, PubMed:30612738).

The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416).

In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
Biological Process
Cell population proliferation Source: Ensembl
Cellular response to DNA damage stimulus Source: UniProtKB
DNA double-strand break processing Source: Reactome
DNA duplex unwinding Source: BHF-UCL
DNA recombination Source: ProtInc
DNA repair Source: ProtInc
DNA strand resection involved in replication fork processing Source: UniProtKB
Double-strand break repair Source: ProtInc
Double-strand break repair via homologous recombination Source: UniProtKB
Double-strand break repair via nonhomologous end joining Source: CACAO
Homologous chromosome pairing at meiosis Source: Ensembl
Homologous recombination Source: ComplexPortal
Meiotic DNA double-strand break formation Source: GO_Central
Mitochondrial double-strand break repair via homologous recombination Source: GO_Central
Mitotic G2/M transition checkpoint Source: ComplexPortal
Mitotic G2 DNA damage checkpoint signaling Source: GO_Central
Mitotic intra-S DNA damage checkpoint signaling Source: GO_Central
Negative regulation of apoptotic process Source: BHF-UCL
Positive regulation of kinase activity Source: BHF-UCL
Positive regulation of protein autophosphorylation Source: BHF-UCL
Positive regulation of telomere maintenance Source: BHF-UCL
Reciprocal meiotic recombination Source: ProtInc
Regulation of mitotic recombination Source: ProtInc
Sister chromatid cohesion Source: BHF-UCL
Telomere maintenance Source: GO_Central
Telomere maintenance via telomerase Source: ProtInc
Telomeric 3' overhang formation Source: BHF-UCL
Cellular Location
Nucleus
Other locations
telomere
Chromosome
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.
Involvement in disease
Ataxia-telangiectasia-like disorder 1 (ATLD1):
A rare disorder characterized by progressive cerebellar ataxia, dysarthria, abnormal eye movements, and absence of telangiectasia. ATLD patients show normal levels of total IgG, IgA and IgM, although there may be reduced levels of specific functional antibodies. At the cellular level, ATLD exhibits hypersensitivity to ionizing radiation and radioresistant DNA synthesis.
Defects in MRE11 can be a cause of nephronophthisis-related ciliopathies (NPHP-RC), a group of recessive diseases that affect kidney, retina and brain. A homozygous truncating mutation MRE11 has been found in patients with cerebellar vermis hypoplasia, ataxia and dysarthria.
PTM
Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome.
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For research use only. Not intended for any clinical use.

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