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Mouse Anti-NAAA Recombinant Antibody (3F4) (CBMAB-N1016-WJ)

This product is a Mouse antibody that recognizes NAAA. The antibody 3F4 can be used for immunoassay techniques such as: ELISA, IP, WB.
See all NAAA antibodies
Published Data
Fig.1 Western blot analysis to examine the expression of girdin and p-girdin.
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Fig.2 Phagocytosis of PLTs forces cell invasion and migration abilities.
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Fig.3 Megakaryocytes(CD61+CD41+760 cells) are expressed as percentage on total BM cells absolute number 761 and analysed in the bone marrow of age-matched WT, WKO and CoWas mice.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3F4
Antibody Isotype
IgG2a, κ
Application
ELISA, IP, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
N-acylethanolamine acid amidase
Introduction
This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
27163
UniProt ID
Q02083
Alternative Names
N-Acylethanolamine Acid Amidase; Acid Ceramidase-Like Protein; ASAH-Like Protein; ASAHL; PLT; N-Acylsphingosine Amidohydrolase (Acid Ceramidase)-Like; N-Acylethanolamine-Hydrolyzing Acid Amidase; N-Acylsphingosine Amidohydrolase-Like; EC 3.5.1.-;
Function
Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine> N-myristoylethanolamine> N-lauroylethanolamine = N-stearoylethanolamine> N-arachidonoylethanolamine> N-oleoylethanolamine (PubMed:15655246, PubMed:17980170, PubMed:18793752, PubMed:30301806, PubMed:22825852).

Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine (PubMed:15655246).
Biological Process
Fatty acid metabolic process Source: UniProtKB
Lipid catabolic process Source: UniProtKB-KW
N-acylethanolamine metabolic process Source: UniProtKB
N-acylphosphatidylethanolamine metabolic process Source: UniProtKB
Sphingosine metabolic process Source: UniProtKB
Cellular Location
Lysosome
Other locations
Membrane
PTM
N-glycosylated (PubMed:17980170, PubMed:18793752, PubMed:30301806). Tunicamycin treatment causes a reduction in specific activity against N-palmitoylethanolamine.
A disulfide bond is seen in the crystal structure of the human protein, but the Cys residues are not conserved in rodents.
Autoproteolytic cleavage at pH 4.5 gives rise to the alpha and beta subunit (PubMed:15655246, PubMed:22040171, PubMed:17980170, PubMed:18793752, PubMed:30301806). Cleavage gives rise to a conformation change that activates the enzyme (PubMed:17980170, PubMed:18793752, PubMed:30301806, PubMed:22040171). The same catalytic Cys residue mediates the autoproteolytic cleavage and subsequent hydrolysis of lipid substrates (PubMed:17980170, PubMed:18793752) (Probable).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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